姜黄素对骨关节炎软骨细胞增殖及分泌MMP-13,IL-6的影响

目的研究姜黄素对骨关节炎(OA)患者软骨细胞增殖、细胞因子MMP-13和IL-6分泌的影响。方法收集4例OA患者关节软骨作为实验组,3例股骨头骨折的关节软骨为对照组,分离软骨细胞进行体外培养。使用浓度为0,10,40,80,120μmol/L的姜黄素溶液共培养软骨细胞48 h,MTT检测姜黄素对软骨细胞增殖的作用,ELISA检测共培养细胞上清液中MMP-13和IL-6含量。结果姜黄素在40μmol/L浓度以上对OA软骨细胞增殖具有明显抑制作用(P<0.05),120μmol/L浓度对正常软骨细胞有明显抑制作用(P<0.05);80μmol/L以上浓度对OA软骨细胞和正常软骨细胞分泌MMP-13具有抑制作用(P<0.05);80μmol/L以上浓度对OA软骨细胞分泌IL-6具有抑制作用(P<0.05),120μmol/L浓度时对正常软骨细胞分泌IL-6具有抑制作用(P<0.05)。结论姜黄素能够抑制软骨细胞的细胞增殖,抑制软骨细胞释放MMP-13和IL-6,减轻炎症反应,保护软骨细胞,延缓软骨退变。

Effect of curcumin on proliferation and secretion of MMP-13 and IL-6 of chondrocyte in osteoarthritis

Objective It is to study the effect of curcumin on the cells proliferation and the secretion of MMP-13,IL-6 of chondrocyte in osteoarthritis(OA).Methods Articular cartilage of 4 cases of patients with OA was collected as experimental group,that of 3 cases with femoral head fracture was collected as control group.The chondrocyte were cultured in vitro with curcumin(0,10,40,80 and 120 μmol/L) for 48 h.The proliferation of chondrocytes were analyzed by MTT.The concentration of MMP-13 and IL-6 was measured by ELISA.Results The curcumin could inhibit obviously the proliferation of OA chondrocyte when its concentration was more than 40 μmol/L(P<0.05),and the normal chondrocyte could be inhibited when the concentration was 120 μmol/L(P<0.05).When its concentration was more than 80 μmol/L it could inhibit the section of MMP-13 in OA and normal chondrocyte(P<0.05).The IL-6 in OA chondrocyte was inhibited when the concentration was more than 80 μmol/L(P<0.05),and the normal chondrocyte was inhibited when the concentration was120 μmol/L(P<0.05).Conclusion Curcumin can inhibit the secretion of MMP-13 and IL-6,and inhibit the chondrocyte proliferation,thus to relieve inflammation,protect chondrocyte and delay cartilagedegeneration.