褪黑素对内毒素血症大鼠血清炎性因子的影响

目的探讨褪黑素(MT)对脂多糖(LPS)诱导的内毒素血症大鼠炎性因子肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)和抗炎因子IL-10表达的影响。方法雄性SD大鼠48只,分为溶剂对照组、LPS组、LPS+MT组、MT组,每组12只。检测各组大鼠血清超氧化物歧化酶(SOD)和丙二醛(MDA)的含量;应用酶联免疫吸附法(ELISA)检测血清TNF-α、IL-6和IL-10含量。结果 LPS+MT组的血清SOD活性较LPS组明显增高(P<0.01),MDA含量较LPS组明显降低(P<0.01);LPS组大鼠血清TNF-α,IL-6含量明显上升,与对照组比较差异有统计学意义(P<0.01),而给予LPS前后应用MT干预治疗,可显著降低血清TNF-α、IL-6含量,与同时间点LPS组比较均差异有统计学意义(P<0.01)。结论 MT可以降低内毒素血症大鼠血清炎性因子水平。

Effect of melatonin on the inflammatory factors in serum of rats with endotoxemia

Objective To investigate the influence of melatonin (MT) on the levels of tumor necrosis factor alpha (TNF-α),interleukin 6 (IL-6) and interleukin 10 (IL-10) in serum of rats with endotoxemia.Methods 48 male Sprague-Dawley rats were randomly divided into four groups:solvent control group,LPS group (LPS 4mg/kg,i.p.),LPS+MT group (MT 5mg/ml,i.p.),with 12 rats in each group.The contents of superoxide dismutase (SOD) in serum and malondialdehyde (MDA) were detected,and the levels of TNF-α,IL-6,IL-10 were detected by enzyme linked immunosorbent assay (ELISA).Results The activity of SOD in serum in LPS +MT group was significantly increased,as compared with that in LPS group (P<0.01),however,the content of MDA in LPS +MT group was significantly decreased,as compared with that in LPS group (P<0.01).The levels of serum TNF-α and IL-6 were significantly increased in LPS groups,as compared with those in solvent control group(P<0.01).However the MT intervention after treatment with LPS could obviously reduce the contents of TNF-α and IL-6 in LPS+MT group,as compared with those in LPS group in the same time point(P<0.01).Conclusion MT can reduce the levels of inflammatory factors in serum of rats with endotoxemia.