乳腺癌组织中VEGF的表达与CD44v6、MMP一2的相关性研究

目的检测血管内皮生长因子（VEGF）与CD44v6、MMP-2在乳腺癌中的表达情况，探讨它们之间的关系。方法采用免疫组化SP法检测92例乳腺浸润性癌中VEGF、CD44v6、MMP-2的表达情况，并与正常乳腺组织进行比较。结果VEGF在正常乳腺组织和乳腺癌中的阳性表达率分别为6．7％（3／45）和90．2％（83／92），且VEGF在淋巴结转移组中的阳性表达率（53／55）明显高于无淋巴结转移组（P〈0．05）。CD44v6在正常乳腺组织及乳腺癌中的阳性表达率分别为8．9％（4／45）和85．9％（79／92），而且CD44v6在淋巴结转移组中的阳性表达率（51／55）明显高于无淋巴结转移组（P〈0．05）。MMP-2蛋白在正常乳腺组织及乳腺癌中的阳性表达率分别为4．4％（2／45）、87．0％％（80／92），而且MMP-2在淋巴结转移组中的阳性表达率（52／55）明显高于无淋巴结转移组（P〈0．05）。VEGF与CD44v6、MMP-2的表达均存在正相关关系（P〈0．05）。结论VEGF、CD44v6、MMP-2在乳腺癌组织中高表达，且均与淋巴结转移有关，它们可能在乳腺癌的远处转移中起协同作用。

Relationship and expression of VEGF,CD44v6 and MMP-2 in breast carcinoma

Objective To observe the expression of VEGF,CD44v6 and MMP-2 in invasive breast carcinoma,and to explore the correlation among them.Methods The expressions of VEGF,CD44v6 and MMP-2 were detected in 92 cases of invasive breast carcinoma by SP immunohistochemistry,and were compared with those of normal tissues(control group) .Results The positive expression rates of VEGF in control group and breast carcinoma group were 6.7%(3/45) ,90.2%(83/92) respectively,furthermore the positive expression rate(53/55) of VEGF in lymph node metastasis tissue was significantly higher than that of the tissue without lymph node metastasis(P<0.05) .The positive expression rates of CD44v6 in normal breast tissue and breast carcinoma were 8.9%(4/45) ,85.9%(79/92) respectively,furthermore,the positive expression rate(51/55) of CD44v6 in lymph node metastasis tissue was significantly higher than that of the tissue without lymph node metastasis(P<0.05) .The positive expression rates of MMP-2 protein in normal breast tissue and breast carcinoma were 4.4%(2/45) ,87.0%(80/92) respectively,and the positive rate(52/55) of MMP-2 in lymph node metastasis tissue was significantly higher than that of the tissue without lymph node metastasis(P<0.05) .The expression of VEGF was positively related with that of CD44v6 and MMP-2(P<0.05) .Conclusion VEGF,CD44v6 and MMP-2 are highly expressed in breast carcinoma,which are related with lymph node metastasis,and these factors may have synergistic effect in the lymph node metastasis of breast carcinoma.