| L型结核菌rpoB基因突变与利福平耐药性的关系研究 |
| |
| 引用本文： | 陈俊林,顾德林,戴建忠,施军卫,陈晓丽.L型结核菌rpoB基因突变与利福平耐药性的关系研究[J].临床肺科杂志,2012,17(9):1624-1625. |
| |
| 作者姓名： | 陈俊林 顾德林 戴建忠 施军卫 陈晓丽 |
| |
| 作者单位： | 南通,南通市第六人民医院结核科,江苏,226013 |
| |
| 基金项目： | 2009年南通市科技局社会发展项目科技课题 |
| |
| 摘 要： | 目的探讨L型结核分枝杆菌rpoB基因突变与利福平耐药性的关系。方法对76株复制肺结核患者L型结核分枝杆菌临床分离株进行药敏试验,同时采用PCR和PCR-DS技术对L型结核菌株进行rpoB基因检测和序列分析。结果药敏结果提示28株L型结核分枝杆菌对利福平耐药,其中20株(71.4%)L型结核分枝杆菌rpoB基因发生突变。结论 L型结核分枝杆菌rpoB基因突变是造成结核分枝杆菌形成利福平耐药性的主要机制。
|
| 关 键 词： | 结核分枝杆菌 细胞壁缺陷 rpoB基因 耐药性 |
Relationship of rpoB gene mutation and rifampin resistance in L-forms of Mycobacterium tuberculosis |
| |
| Institution: | CHEN Jun-lin,GU De-ling,DAI Jian-zhong,et al.Department of tuberculosis Medicine,Nantong sixth people’s Hospital,Nantong 226013,China |
| |
| Abstract: | Objective To study the relationship of rpoB gene mutation and rifampin resistance in L-forms of Mycobacterium tuberculosis.Methods Sensitivity test to rifampin in 76 clinical isolated strains from L-forms of Mycobacterium tuberculosis patients was made,and the rpoB genes and sequence analysis were detected by PCR and PCR-DS methods.Results 28 clinical isolated strains from L-forms of Mycobacterium tuberculosis were resistant to rifampin,and the rpoB gene of 20 strains had mutation.Conclusion The rpoB genes mutation in L-forms of Mycobacterium tuberculosis was the main mechanism of rifampin resistance of Mycobacterium tuberculosis. |
| |
| Keywords: | mycobacterium tuberculosis cell wall deficient RPOB gene drug resistance |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
