间歇低氧幼鼠8-异前列腺素F2α变化及芹菜素的干预效果

目的：观察间歇低氧幼鼠血清及海马8-异前列腺素F2α（8-ISO—PGF2α）的变化及芹菜素的干预效果。方法：取3～4周龄健康雄性SPF级SD幼鼠32只，随机分为间歇低氧组（IH）、空气对照组（C）、芹菜素十二甲基亚矾组（Q）和二甲基亚砜组（R），每组8只，以间歇低氧舱建立间歇低氧幼鼠模型，99．99％的纯氛和99．50％的医用氧交替输入舱内，90s为一次低氧-复氧循环；间歇低氧2周后，ELISA法测定各组血清及海马8-ISO—PGF2α水平。结果：血清中，IH组和R组8-ISO—PGF2α的含量分别高于C组和Q组（均P〈0．01），而C组与Q组比较差异无显著性（均P〉0．05），IH组与R组比较差异无显著性（均P〉0．05）；在海马中，结果类似；血清和海马组织中8-ISO—PGF2α含量呈正相关（r=0．818，P〈0．01）。结论：间歇低氧后幼鼠血清及海马中8-ISO—PGF2α表达均升高，芹菜素可降低血清和海马中的8-ISO—PGF2α水平，在治疗间歇低氧造成的损伤中具有应用前景。

Changes of 8-iso-prostaglandin F2αlevel in immature rats exposing to intermittent hypoxic and The intervention effect of apigenin.

Objective： To detect the changes of 8-iso-prostaglandin F2α （8-ISO-EGF2α） in serum and the hippocampus and to observe the intervention effect of apigenin in immature rats exposing to intermittent hypoxic. Methods： Thirty two 3-4 week-aged SDrats were randomly divided into intermittent hypoxia （IH） group,air control（C） group,apigenin＋ DMSO （Q） group and DMSO control（R） group. Each group had eight rats. Intermittent hypoxia model was induced by an intermittent hypoxia cabin and the cabin was flushed with 99.99% nitrogen alternating with 99.5% oxygen. The cycle of hypoxia- reoxygenation was 90 s. After 2 weeks, the serum and hippocampus levels of 8-ISO-Prostaglandin F were tested by ELISA. Results： In the serum, the expression of 8-ISO-FGF in IH and R groups were significant higher than that in C and Q groups （F〈0.01, respectively）. There were no difference between C and Q groups （P〉0.05）, and no difference between IH and R groups （P〉0.05）. The similar results were found in hippocampus. Serum 8-ISO-PGF2α and hippocampus 8-ISO-PGF were positively correlated （r =0.818, P〈0.01）. Conclusion： The levels of 8-iso-PGF2α, increases in both serum and hippocampus of immature rats with IH and the levels of 8-iso-FGF in serum can reflect indirectly the degree of oxidative stress in hippocampus. Apigenin can reduce the level of 8-iso-PGF in serum and hippocampus. Therefore, it has plication prospect in treatment of injuries which induced by IH.