1,25-(OH)2 D3在大鼠肾移植急性排斥反应中的作用

目的 观察1,25-(OH)2D3在大鼠肾移植急性排斥反应中的作用,探讨其在免疫调节中的机制.方法 行Wistar为供体,SD大鼠为受体的肾移植术,24只SD大鼠随机分为4组,检测血清中尿素氮(BUN)、肌酐(Cr)、Ca2+、P3+、白细胞介素(IL)-2、干扰素(IFN)-γ的水平及移植肾的病理变化.结果 Ⅰ组大鼠肾移植后5 d血清中BUN、Cr、IL-2、IFN-γ水平分别明显高于Ⅱ、Ⅲ、Ⅳ组(P＜0.05),Ⅱ、Ⅲ组之间比较差异无统计学意义(P＞0.05),但分别与Ⅳ组比较均明显增高(P＜0.05).各组Ca2+、P3+比较差异无统计学意义(P＞0.05).病理结果 显示,Ⅰ组大鼠移植后肾脏排斥反应最重,其余组与之比较均有所减轻,以Ⅳ组最明显.结论 1,25-(OH)2D3通过减少IL-2、IFN-γ的产生,减少排斥反应的发生,改善移植肾的功能.

Effects of 1,25-dihydroxyvitamin D3 in acute rejection of rat renal transplantation

Objective To study the effects of 1,25-dihydroxyvitamin D3(1,25-(OH)2D3)on acute rejection of rat renal transplantation and the mechanism of its immunoloregulation.Methods Kidney transplantation from Wismr rats to SD rats was used as a model of study.Twenty-four SD rats were divided into 4 groups randomly.The levels of BUN,Cr,Ca2+,P3+,IL-2,and IFN-γ in serum were determined.HE staining inspected all specimens of kidney grafts.Results The levels of BUN,Cr,IL-2,and IFN-γ in the group Ⅰ were lower than those in groups Ⅱ,Ⅲ,Ⅳ(P＜0.05),and there was no significant difference in BUN,Cr,IL-2,and IFN-γ between groups Ⅱ andⅢ(P＞0.05),but they were lower in groups Ⅱ and Ⅲ than in group Ⅳ(P＜0.05).The levels of Ca2+ and P3+ in serum had no signigicant difference among groups.The obvious histological change of rejection in grafts were observeed in group Ⅰ.and that Was alleviated in the other groups,especially in group Ⅳ.Conclusion 1,25-(OH)2D3 reduced the acute reiection by decreasing the production of IL-2 and IFN-γ,and ameliorated the function of kidney grafts.