COX-2在肾母细胞瘤中的表达及意义

目的通过研究COX-2在肾母细胞瘤中的表达，探讨COX-2与肾母细胞瘤的关系。方法应用免疫组织化学SABC法柃测25例肾母细胞瘤患儿肿瘤组织标本及10例瘤旁肾组织标本（距肿瘤边缘2cm的肾组织标本）中COX-2的表达情况。结果COX-2在肾母细胞瘤中阳性表达率为84％，对照组阳性表达率为10％，两者比较，差异有统计学意义（χ^2=16．753，P〈0．001）。伴有淋巴结转移的肾母细胞瘤患儿淋巴结COX-2阳性表达率高于无淋巴结转移者（χ^2=9．000,P〈0．01）。COX-2的阳性表达率随临床分期的增加而增高。COX－2的阳性表达率与肾母细胞瘤的临床分期呈正相关（r=0．966，P〈0．05）。结论COX－2的阳性表达率有随肾母细胞瘤临床分期的升高而增高的趋势，提示COX-2可能参与了肾母细胞瘤的发生发展过程，可为肾母细胞瘤的治疗和预防开辟新的途径。

Expression of Cyclooxygenase-2 in Wilms Tumor

Objective To investigate the protein expression of cyclooxygenase-2 （COX-2） in Wilms tumor and find the relationship between cyclooxygenase-2 expression and Wilms tumor. Methods 25 cases of Wilms tumor were studied. Ten controls were obtained from peritumor normal tissues After serial slicing to 4 μm the histological slides were stained with hematoxylin and eosin, and immunostained with anti-cyclooxygenase-2 antibodies. Immunostaining was graded semiquantitatively according to the percent of stained cells with the cytoplasmic pattern of staining and according to staining intensity. Results The positive expression rate of COX-2 was 84% in Wilms tumor and 10% in peritumor normal tissues （χ^2=16. 753,P 〈 0. 001）. Expression was also observed in Wilms tumor lymphaden metastasis. In comparison, cyclooxygenase-2 expression in Wilms tumor which didn＇t have lymphaden metastasis was less prominent. ）. The correlation between COX-2 expression and clinical stage of Wilms tumor was significant in statistics （r = 0.966, P 〈 0.05 ）. Conclusions Cyclooxygenase-2 expression is characteristic of all Wilms tumors at all stages. Aberrant COX-2 expression may play an important role in the occurrence of Wihns tumors.