| Tca8113细胞系下颌下淋巴结转移动物模型的建立 |
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| 引用本文： | 余杨,邱蔚六,吕燕,叶冬霞,郭伟.Tca8113细胞系下颌下淋巴结转移动物模型的建立[J].口腔颌面外科杂志,2009,19(3):159-163. |
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| 作者姓名： | 余杨 邱蔚六 吕燕 叶冬霞 郭伟 |
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| 作者单位： | 上海交通大学医学院附属第九人民医院口腔颌面外科 上海市口腔医学研究所,上海市口腔医学重点实验室,上海,200011 |
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| 基金项目： | 上海交通大学科技基金，国家自然科学基金，上海市科委重点课题，上海人才发展基金，上海市重点学科(优势学科)建设资助项目 |
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| 摘 要： | 目的:建立人口腔鳞癌Tca8113裸鼠舌体及颊黏膜移植瘤淋巴结转移模型,研究分析两种转移模型的特性。方法:通过在裸鼠舌体及颊黏膜内注射接种Tca8113细胞,建立移植瘤动物模型。舌体及颊部原发灶分别在2周和4周时被切除,观察6周时下颌下淋巴结转移情况。通过组织切片HE染色法计算淋巴结转移率,并采用CK蛋白免疫组织化学染色方法计算淋巴结微灶转移率。结果:13只舌体接种移植瘤裸鼠中,有3只出现下颌下淋巴结转移,5只出现淋巴结微灶转移,转移率达到61.54%。15只颊黏膜接种移植瘤裸鼠中,3只出现下颌下淋巴结转移,3只出现淋巴结微灶转移,转移率为40.00%。结论:裸鼠舌体及颊黏膜接种Tca8113建立的转移模型具有一定的转移率,并能较好地模拟临床口腔鳞癌的转移,为实验研究提供了良好的动物模型。
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| 关 键 词： | 鳞状细胞癌 淋巴结 转移 动物模型 |
Nude Mice Metastasis Model of Human Oral Squamous Carcinoma Constructed Using Tca8113 Cell |
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| YU Yang,QIU Wei-liu,L Yan,YE Dong-xia,GUO Wei.Nude Mice Metastasis Model of Human Oral Squamous Carcinoma Constructed Using Tca8113 Cell[J].Chinese Journal of Oral and Maxillofacial Surgery,2009,19(3):159-163. |
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| Authors: | YU Yang QIU Wei-liu L Yan YE Dong-xia GUO Wei |
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| Institution: | Department of Oral and Maxillofacial Surgery;Shanghai Ninth People's Hospital;School of Medicine;Shanghai Jiaotong University;Shanghai Research Institute of Stomatology;Shanghai Key Laboratory of Stomatology;Shanghai 200011;China |
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| Abstract: | Objective:To establish two metastasis nude mice models from different cancer locations with Tca8113 cell and clarify their metastasis characters.Methods:Transplantation tumor models of nude mice were established by injecting Tca8113 cells into tongue or buccal mucosa.Transplantation tumors of tongues were removed after 2 weeks and those on buccal mucosa were removed after 4 weeks.6 weeks later,submandibular lymph nodes were examinated under microscopy.Immunohistochemistry examination was applied to detect m... |
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| Keywords: | squamous cell carcinoma lymph node metastasis animal model |
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