黄芪注射液对高糖腹透液作用下人腹膜间皮细胞AQP-1表达的影响

目的:探讨高糖腹透液对人腹膜间皮细胞(HPMC)水通道蛋白1(AQP-1)表达的影响及黄芪注射液的干预作用。方法:采用HPMC体外培养模型,将HPMC分为5组:空白组、模型组、黄芪高、中、低剂量组。分别培养6、24、48h。Western blot法测定AQP-1蛋白的表达;RT-PCR检测24h时间段AQP-1 mRNA的表达。结果:Western Blot结果显示:给药6h后各组AQP-1蛋白表达无明显差异;24h后发现黄芪高、中剂量组能明显上调AQP-1蛋白表达,48h后发现仅黄芪高剂量组能上调AQP-1蛋白表达。RT-PCR结果显示:与模型组相比黄芪高、中、低剂量组AQP-1 mRNA的表达均明显上调(P<0.01),黄芪高剂量组作用更显著。结论:黄芪注射液能上调高糖环境下HPMC AQP-1的表达。

Effect of Astragalus injection on the expression of aquaporin-1 of human peritoneal mesothelial cell in high glucose dialysate

Objective: To investigate the expression of aquaporin-1 of Human peritoneal mesothelial cell in High glucose dialysate and observe the intervention of Astragalus injection.Methods: The use of HPMC in vitro model,We divided HPMC into five groups: The control group,Model group,AG High-dose group,AG middle-dose group,AG Low-dose group.After cultured 6,24,48h,we extracted cell protein and using Western blot tested the expression of AQP-1.and using RT-PCR tested the expression of AQP-1mRNA in 24h.Results:Western blot results showthat there is no significant difference of AQP-1 protein expression in each group after 6h administration.after 24h we find Astragalus high dose group and the middle-dose group could significantly up-regulate the expression of AQP-1 protein;after 48h we only find the high-Astragalus group can increases AQP-1 protein expression.RT-PCR results show that compared with model group,the expression of AQP-1 mRNA of three AG dose group were significantly increased,the high-Astragalus dose group increased more significantly.Conclusion: Astragalus injection can increase the expression of HPMC AQP-1 in high glucose dialysate,which is related with time.