| HPLC-MS法测定大鼠血浆中双-O-甲基四氢呋喃愈创木素B的浓度及药代动力学参数 |
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| 引用本文： | 徐春蕾,刘史佳,居文政,陈勇,张芳,李祥.HPLC-MS法测定大鼠血浆中双-O-甲基四氢呋喃愈创木素B的浓度及药代动力学参数[J].中国药理学通报,2012,28(12):1766-1769. |
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| 作者姓名： | 徐春蕾 刘史佳 居文政 陈勇 张芳 李祥 |
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| 作者单位： | 1. 南京中医药大学药学院,江苏,南京,210023
2. 南京中医药大学附属医院临床药理科,江苏,南京,210029 |
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| 基金项目： | 国家自然科学基金青年项目，江苏省高校自然科学研究项目，江苏省普通高校研究生科技创新计划，南京中医药大学校青年自然科学基金项目 |
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| 摘 要： | 目的建立测定大鼠血浆中双-O-甲基四氢呋喃愈创木素B(DiB)含量的高效液相色谱-电喷雾离子化-质谱(HPLC-ESI-MS)联用的分析方法,并用于其在大鼠体内的药代动力学研究。方法 SD大鼠6只,单剂量静脉注射(10mg.kg-1)DiB,以三白草酮为内标,用HPLC-MS法测定给药后血浆中的药物浓度,并用DAS 2.0软件计算药动学参数。结果 DiB的血药浓度在0.025～5.0 mg.L-1范围内线性关系良好,最低定量限为0.025 mg.L-1,提取回收率≥91.1%,日间、日内RSD≤10.0%。大鼠单剂量尾静脉注射DiB 10 mg.kg-1后,主要动力学参数AUC(0-t)、T12β、CL、V1分别为:(11.44±2.44)mg.h.L-1、(3.73±1.30)h、(0.65±0.14)L.h-1.kg-1、(3.36±3.19)L.kg-1。结论该方法操作简便、灵敏、快速、专属性强,可用于DiB的血药浓度检测及药代动力学研究。
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| 关 键 词： | 双-O-甲基四氢呋喃愈创木素B HPLC-MS 药代动力学 血药浓度 大鼠 静脉注射 |
Determination of di-O-methyltetrahydrofuriguaiacin B in rat plasma by HPLC-MS for pharmacokinetic studies |
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| XU Chun-lei
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LIU Shi-jia
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JU Wen-zheng
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CHEN Yong
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ZHANG Fang
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LI Xiang.Determination of di-O-methyltetrahydrofuriguaiacin B in rat plasma by HPLC-MS for pharmacokinetic studies[J].Chinese Pharmacological Bulletin,2012,28(12):1766-1769. |
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| Authors: | XU Chun-lei LIU Shi-jia JU Wen-zheng CHEN Yong ZHANG Fang LI Xiang |
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| Institution: | 1(1.Nanjing University of Traditional Chinese Medicine,Nanjing 210023,China;2.Dept of Clinical Pharmacology,the Affiliated Hospital of Nanjing University of Traditional Chinese Medicine,Nanjing 210029,China) |
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| Abstract: | Aim To establish a HPLC-ESI-MS method for determination of DiB in plasma and its application to rats.Method Six Sprague-Daw ley rats were i.v.administrated 10 mg·kg-1 of DiB,respectively.Drug plasma concentration was determined by HPLC-MS using sauchinone as an internal standard.The pharmacokinetic parameters were calculated by DAS 2.0.Results Calibration curves were linear over 0.025~5.0 mg·L-1 and LLQD was 0.025 mg·L-1.The recovery was higher than or equal to 91.1% and the RSD of inter-day was less than or equal to 10.0%.After i.v.administration of 10 mg·kg-1 of DiB,the pharmacokinetic parameters of AUC(0-t),T1 2β,CL,V1 were(11.44±2.44) mg·h·L-1,(3.73±1.30) h,(0.65±0.14)L·h-1·kg-1,and(3.36±3.19)L·kg-1,respectively.Conclusion This method is rapid,sensitive,and specific for the determination of DiB in rats. |
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| Keywords: | Di-O-methyltetrahydrofuriguaiacin B HPLC-MS pharmacokinetics plasma concentration rat i v administration |
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