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| 南瓜蛋白联合吉非替尼对人胰腺癌BxPC-3细胞的抑制作用 | |
| 引用本文： | 谢捷明,杨爱琴,王丛菲,黄鹤光,张宝明,吴雪燕,陈明晃. 南瓜蛋白联合吉非替尼对人胰腺癌BxPC-3细胞的抑制作用[J]. 中国药理学通报, 2012, 28(12): 1724-1728 |
| 作者姓名： | 谢捷明 杨爱琴 王丛菲 黄鹤光 张宝明 吴雪燕 陈明晃 |
| 作者单位： | 1. 福建医科大学药学院药理学系,福建,福州,350004 2. 福建医科大学附属协和医院基本外科,福建,福州,350001 3. 中国科学院福建物质结构研究所,结构化学国家重点实验室,福建,福州,350002 |
| 基金项目： | 国家自然科学基金资助项目，福建省自然科学基金资助项目，福建医科大学重大科研项目 |
| 摘 要： | 目的研究南瓜蛋白(cucurmosin,CUS)联合表皮生长因子受体(epidermal growth factor receptor,EGFR)酪氨酸激酶抑制剂吉非替尼(Gefitinib,GEF)对人胰腺癌细胞株Bx-PC-3的生长抑制作用。方法采用SRB法测定CUS与GEF联用对体外培养的BxPC-3细胞的增殖抑制作用;应用集落形成实验测定CUS与GEF联用对体外培养的BxPC-3细胞集落形成的影响;以Western blot技术检测CUS与GEF联用对体外培养的BxPC-3细胞EGFR的影响。结果 CUS联用GEF对BxPC-3细胞的增殖抑制率大于单用药物,q>0.85;CUS联用GEF对BxPC-3细胞的集落形成抑制率大于单用药物,q>1.15;CUS联合GEF干预BxPC-3细胞可明显下调其EGFR蛋白表达,q>2.0。结论 CUS联合GEF对人胰腺癌BxPC-3细胞的生长抑制具有协同作用。 |
| 关 键 词： | 南瓜蛋白 吉非替尼 胰腺癌 EGFR 细胞增殖 药物协同作用 |
Anti-proliferation effect of cucurmosin with the combination of gefitinib on human pancreatic cancer cell line BxPC-3 in vitro |
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| XIE Jie-ming , YANG Ai-qin , WANG Cong-fei , HUANG He-guang , ZHANG Bao-ming , Wu Xue-yan , CHEN Ming-huang. Anti-proliferation effect of cucurmosin with the combination of gefitinib on human pancreatic cancer cell line BxPC-3 in vitro[J]. Chinese Pharmacological Bulletin, 2012, 28(12): 1724-1728 | |
| Authors: | XIE Jie-ming YANG Ai-qin WANG Cong-fei HUANG He-guang ZHANG Bao-ming Wu Xue-yan CHEN Ming-huang |
| Affiliation: | 1.Dept of Pharmacology,Fujian Medical University,Fuzhou 350004;2.Dept of General Surgery, Union Hospital,Fujian Medical University,Fuzhou 350001;3.State Structural Chemistry Key Laboratory of Fujian Institute of Research on the Structure of Matter,Chinese Academy of Sciences,Fuzhou 350002) |
| Abstract: | Aim To investigate the effects of cucurmosin(CUS) combined with EGFR Tyrosine Kinase Inhibitor,gefitinib(GEF),on human pancreatic cancer cells.Methods SRB assay was used to detect proliferation inhibition in human pancreatic cancer cell lines BxPC-3 in vitro after the treatment of CUS combined with GEF.Colony forming assay was used to observe the colony forming efficiency in BxPC-3 cells treated by CUS combined with GEF.Western blot was used to analyze the proteins expressions of EGFR in BxPC-3 cells after the treatment of CUS combined with GEF.Results The inhibition rate of CUS combined with GEF was higher than CUS or GEF alone,q>0.85.The colony formation inhibition rate of the combined treatment group was higher than the groups of CUS or GEF alone,q>1.15.The combined treatment group down-regulated EGFR protein significantly,q>2.0.Conclusion CUS combined with GEF shows a synergistic effect on cells growth inhibition in BxPC-3 cells in vitro. |
| Keywords: | cucurmosin gefitinib pancreatic cancer EGFR cell proliferation synergistic effect |
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