粉防己碱对人结肠癌细胞增殖的影响

目的 :观察粉防己碱对人结肠癌细胞 H T2 9增殖的影响 ,并初步探讨其作用机制。方法 :采用流式细胞仪分析细胞周期变化 ,并测定细胞内游离钙离子浓度水平 ;MTT比色法测定细胞增殖情况。结果 :2～ 10 μg/m L 的粉防己碱 (Tet)能明显抑制 HT2 9细胞的增殖 (P<0 .0 5 ) ,其半数抑制浓度 (IC5 0 )约为 8μg/m L。 8μg/m L 的 Tet可以使细胞内游离钙离子浓度 (〔Ca2 +〕i)水平明显降低 (P<0 .0 1)。不同浓度的 Tet可使 HT2 9细胞 C1 期或 G2 + M期明显增加 ,S期细胞明显减少 (P<0 .0 1)。结论 :粉防己碱通过抑制钙离子信号传递途径 ,使细胞周期进行受阻 ,可能是其抑制 HT2 9细胞增殖的一个主要机制。

Effects of Tetrandrine on Proliferation of Human Colon Carcinoma Cells

Objective: It is to study the effects of tetrandrine(Tet)on proliferation of HT29 colon carcinoma cells and investigate the mechanism. Methods: Flow cytometry was used to analyze the cell cycles and monitor the cytoplasmic free calcium (〔Ca 2+ 〕i). MTT colorimetry was used to determine the proliferation of HT29 cells. Results: 2~10μg/mL Tet could significantly inhibit the proliferation of HT29 cells ( P <0.05). Half-maximal inhibition of growth was observed at concentration of 8μg/mL Tet. 8μg/mL Tet can significantly decrease the level of 〔Ca 2+ 〕i in HT29 cells ( P <0.01). Tet can increase the percentages of G 1 and G 2+M phases in HT29 cell cycles ( P <0.01), and decrease the percentages of the S phase ( P <0.01). Conclusion: The inhibition of calcium signal transduction by Tet might be responsible for the antiproliferation of Tet HT29 colon carcinoma cells.