针刺对糖尿病小鼠脑内NOS表达的影响

目的 :本研究探讨一氧化氮 (NO)在糖尿病神经病变发病机制中的作用及针刺的效应。方法 :用腹腔注射链脲佐菌素 (Streptozotocin ,STZ)建立糖尿病动物模型 ,采用免疫组织化学方法观察糖尿病小鼠脑内神经原性一氧化氮合酶 (nNOS)免疫阳性神经元在脑内的分布。结果 :糖尿病可引起小鼠脑内各区 (包括大脑皮层、海马、下丘脑和杏仁核 )nNOS免疫阳性神经元表达增多 ,大脑皮层和杏仁核内的nNOS表达增加有显著性意义 ,在杏仁核和梨状皮质过度区也有大量nNOS阳性神经元分布 ,而针刺能抑制糖尿病引起的nNOS免疫阳性神经元表达的增加 ,这种抑制作用在大脑皮层和杏仁核具有显著性意义。另外在糖尿病小鼠的海马、下丘脑中可见到nNOS阳性神经元 ,也较正常组多 ,针刺右侧“太溪”对nNOS阳性神经元的增加也有一定的抑制作用 ,但均未达显著性意义。结论 :糖尿病可增加脑内nNOS免疫阳性神经元表达 ,针刺可抑制这种nNOS阳性神经元的增加 ,有类似NOS抑制剂的作用 ,可拮抗nNOS的神经毒性

Effect of Acupuncture on Nitric Oxide Synthase Expression in the Brain of Diabetes Mice

Objective: To explore the effect of nitric oxide (NO) in the pathogenesis of diabetic neuropathy and the effect of acpuncture on nitric oxide synthase (NOS) expression in the brain. Methods: Twenty one Kunming male mice wererandomly divided into normal control group (n=6), diabetes group (n=7) and acupuncture group (n=8). NOS expression was displayed using immunohistochemical technique. Diabetes mouse model was produced by intraperitoneal injection of streptozotocin (STZ). Right "Taixi" (KI 3) was punctured and stimulated by twirling the acupuncture needle continuously for 2 min with hand. The treatment was given once every other day, continuously for 9 sessions. Results: After inducing diabetes for 6 weeks, the positive neurons of nNOS in different regions (cerebral cortex, hippocampus, hypothalamus, amygdaloid nucleus, the transitional area between the amygdala and the piriform cortex, the basolateral nucleus and the anterior region of the amygdala) of the brain increased in diabetes group than those of normal control group, especially being significant in the cerebral cortex and amygdaloid nucleus (P<0.01). But in acupuncture group, the positive neurons in the above mentioned brain regions particularly in cerebral cortex and amygdala were fewer in number than those of diabetes group.(P<0.01). Conclusion: In diabetes mice, acupuncture may suppress nNOS expression that mimics the effect of NOS antagonists in resisting nNOS toxicity.