| 一种改进的大鼠脑组织灌注固定方法 |
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| 引用本文： | 陈浩宇,高亚兵,彭瑞云,王德文,刘杰. 一种改进的大鼠脑组织灌注固定方法[J]. 军事医学科学院院刊, 2002, 26(3): 208-209,212 |
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| 作者姓名： | 陈浩宇 高亚兵 彭瑞云 王德文 刘杰 |
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| 作者单位： | 军事医学科学院放射医学研究所,北京,100850 |
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| 摘 要： | 目的：改进常规脑灌注固定方法存在着一些不足，使其组织结构更佳，提高灌注效率。方法：改进灌注针头和灌注液，改变灌注的循环通路。结果和结论：提高了灌注效率，节省灌注时间和灌注液，结构更加清晰，结果可靠。为有关脑组织的各种常规病理形态、免疫组化、原位杂交、原位PCR的实验研究提供了一种更加简便、快捷、有效可行的方法。
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| 关 键 词： | 脑 大鼠 免疫组织化学 聚合酶链反应 灌注固定 |
| 文章编号： | 1000-5501(2002)03-0208-03 |
An improved method for affusion fixation of rats brain |
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| CHEN Hao_Yu,GAO Ya_Bing,PENG Rui_Yun,WANG De_Wen,LIU Jie. An improved method for affusion fixation of rats brain[J]. Bulletin of the Academy of Military Medical Sciences, 2002, 26(3): 208-209,212 |
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| Authors: | CHEN Hao_Yu GAO Ya_Bing PENG Rui_Yun WANG De_Wen LIU Jie |
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| Abstract: | Objective:To improve the affusion fixation method by overcoming several shortages of the routine method.Methods:The circle route of affusion, the affusing syringe needle and solution were improved.Results:The new method has some advantages: enhancing the efficiency, saving time, saving the solution, showing clear structure, and giving reliable results.Conclusions: The new method provides a more simple and effective method for the experiment on brain, including H.E.staining, immunocytochemistry, in situ hybridization, and in situ PCR. |
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| Keywords: | brain rat immunohistochemistry polymerase chain reaction affusion fixation |
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