家族性腺瘤性息肉病中VHL和VEGF的表达

目的:分析希佩尔-林道病(VHL)蛋白和血管内皮生长因子(VEGF)在家族性腺瘤性息肉病中的表达,并探讨其在腺瘤形成以及癌变过程中的可能作用机制。方法:收集我科2003年3月到2007年8月10例家族性腺瘤性息肉患者手术切除标本,分别取正常肠黏膜组织、≥1cm的腺瘤性息肉以及癌变腺瘤组织,采用SP法免疫组化方法检测VHL和VEGF在这些标本组织中的表达。结果:VHL阳性信号呈棕黄色主要表达于胞质内,VEGF阳性表达为棕黄色,主要表达于肿瘤细胞胞质。VHL在正常肠黏膜组织和≥1cm的腺瘤性息肉中的表达均较强,两者相差并不显著(χ2=2.4,P>0.05),但明显高于VHL基因在癌变腺瘤中的表达,差异具有统计学意义(χ2=7.2,P<0.05;χ2=14.4,P<0.05);VEGF在正常肠黏膜细胞、≥1cm的腺瘤性息肉到癌变腺瘤组织中阳性表达率不断升高,差异具有统计学意义(χ2=5.051,-P<0.05;χ2=11.52,P<0.05)。结论:VHL可能作为肿瘤抑制基因参与了家族性腺瘤性息肉的发展,VHL和VEGF血管内皮生长因子的异常表达与腺瘤到癌变的过程密切相关。

Expression of VHL Protein and VEGF in Patients with Familial Adenomatous Polyposis

Objective: To analyze the expression of Von Hippel-Lindau(VHL) protein and vascular endothelial growth factor(VEGF) in patients with familial adenomatous polyposis(FAP) and discuss the possible mechanism of VHL protein and VEGF in adenoma formation and cancerization.Methods: The surgical resection specimens were obtained from 10 FAP patients from March 2003 to August 2007,the normal intestinal mucosa,≥1 cm adenomas and cancerous adenomas were subjected to SP immunohistochemistry for analysis of the expression of VHL protein and VEGF in these tissue samples.Results: VHL protein and VEGF was mainly detected in the cytoplasm of tumor cells.The expression of VHL protein in the normal intestinal mucosa was not different from that in ≥1 cm adenoma(P>0.05),but the VHL expression both in normal and in ≥1 cm adenoma tissue were higher than that in the cancerous adenoma cells(both P<0.05).The positive expression rate of VEGF in the normal intestinal mucosa,≥1 cm adenoma tissue and cancerous adenoma increased from low to high(P<0.05).Conclusion: VHL may conduct as a tumor suppressor gene involved in the development of FAP,the abnormal expression of VHL protein and VEGF has closely related to the process of adenomatous cancerization.