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目的 比较微小RNA（miRNAs）在人牙髓干细胞（dental pulp stem cells， DPSCs）及非DPSCs中的表达差异，探讨其在维持DPSCs干性状态中的作用。方法 本研究于2013年1—10月在福建医科大学附属口腔医院完成。 原代培养人牙髓细胞，利用结合了STRO-1特异性抗体的免疫磁珠分选获得DPSCs，并进行成牙本质样细胞的诱导分化，检测碱性磷酸酶（ALP）、骨钙素（OC）值以及进行von Kossa染色，鉴定其分化能力。采用miRNA基因芯片技术，检测DPSCs和非DPSCs中miRNAs的表达，筛选出差异表达的miRNAs。结果 与非DPSCs相比，DPSCs中表达上调超过2倍的miRNAs有11个，表达下调超过2倍的miRNAs有3个。结论 miRNAs表达谱的变化可能与DPSCs干性状态的维持相关。

Comparison of miRNAs expression profiles between human dental pulp stem cells and non-stem cells.

Objective To observe the expression of miRNAs between human dental pulp stem cells （DPSCs） and human dental pulp non-stem cells and to analyze the roles of miRNA in the stemness of DPSCs. Methods DPSCs were isolated by immune-magnetic beads binding specific STRO-1 antibody separation system. Mineralization medium-induced odontoblasts-like cells differentiated from DPSCs were tested by ALP, OC values and von Kossa staining. Differential miRNAs of human dental pulp stem cells and non-stem cells were screened by microRNA array. Results miRNA microarrays revealed that 11 miRNAs were upregulated in DPSCs while 3 miRNAs were downregulated compared with non-stem cells. Conclusion Stemness of DPSCs may have a close relationship with differential miRNAs expression.