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| 神经肽Y经Ca2+/CaM依赖的钙调神经磷酸酶途径诱导心肌细胞肥大 | |
| 引用本文： | 李晓云,陈敏生,黄少华,董颀,李荧辉,张舒,刘振秀.神经肽Y经Ca2+/CaM依赖的钙调神经磷酸酶途径诱导心肌细胞肥大[J].南方医科大学学报,2008,28(12):2139-2141. |
| 作者姓名： | 李晓云 陈敏生 黄少华 董颀 李荧辉 张舒 刘振秀 |
| 作者单位： | 1. 广州医学院第二附属医院心内科,广东,广州,510260 2. 广州医学院第一附属医院,广东,广州,510120 3. 广州医学院生理教研室,广东,广州,510120 4. 航天医学工程研究所,北京,100094 5. 第四军医大学生理系,陕西,西安,710033 |
| 基金项目： | 国家自然科学基金 ， 广东省自然科学基金 |
| 摘 要： | 目的 观察神经肽Y(NPY)诱导心肌细胞肥大效应,及Ca2+/CaM依赖的钙调神经磷酸酶(CaN)途径在其中起的作用.方法 用NPY(10、100nmol)刺激心肌细胞,用环胞索A(CsA)特异性抑制CaN活性.测定心肌细胞蛋白合成速率(3H-Leu掺入量)、CaN-蛋白表达、c-jun mRNA表达、CaN酶活性、细胞内游离钙含量.结果 (1)在100nmol NPY作用下,心肌细胞3H-Leu掺入量及c-jun mRNA表达量均较对照组显著增高(P<0.05,P<0.001).NPY+CsA组和对照组相比无显著差别.(2)在100 nmol NPY作用下,NPY组心肌细胞内CaN酶比活力、CaN-α表达、胞浆及核内钙含量较对照组显著增高(P<0.05,P<0.05,P<0.001,P<0.001).结论 NPY可刺激心肌细胞肥大,CaN信号途径在其中起重要作用. |
| 关 键 词： | 神经肽Y 钙调神经磷酸酶 胞内钙 心肌细胞肥大 |
Role of Ca~(2+)/calmodulin-dependent calcineurin signaling pathway in neuropeptide Y-induced cardiac hypertrophy in rats |
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| LI Xiao-yun,CHEN Min-sheng,HUANG Shao-hua,DONG Qin,LI Ying-hui,ZHANG Shu,LIU Zhen-xiu.Role of Ca~(2+)/calmodulin-dependent calcineurin signaling pathway in neuropeptide Y-induced cardiac hypertrophy in rats[J].Journal of Southern Medical University,2008,28(12):2139-2141. | |
| Authors: | LI Xiao-yun CHEN Min-sheng HUANG Shao-hua DONG Qin LI Ying-hui ZHANG Shu LIU Zhen-xiu |
| Institution: | LI Xiao-yun1,CHEN Min-sheng1,HUANG Shao-hua2,DONG Qin3,LI Ying-hui4,ZHANG Shu5,LIU Zhen-xiu4 1Department of Cardiology,Second Affiliated Hospital of Guangzhuo Medical College,Guangzhou,510260,2First Affiliated Hospital,3Department of Physiology,Guangzhou Medical College,4Institute of Space Medico-Engineering,Beijing 100094,China,5Department of Physiology,Forth Military Medical University,Xi\'an 710033 |
| Abstract: | Objective To investigate the role of Ca2+/calmodulin-dependent calcineurin (CaN) signaling pathway in neuropeptide Y (NPY)-induced cardiomyocyte hypertrophy in rat. Methods Cardiomyocytes of neonatal Wistar rats were cultured in the presence of 10 and 100 nmol/L NPY, and cyclosporine A (CsA) was applied to inhibit the activity of CaN. The protein synthesis rate, c-jun mRNA expression, CaN protein expression, CaN activity and intracellular Ca2+ concentration in the cardiomyocytes were assessed. Results Compa... |
| Keywords: | neuropeptide Y calcineurin intracellular Ca2+ concentration cardiomyocytes hypertrophy |
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