内支架法原位端-端吻合大鼠移植肾静脉

目的 建立一种省时、高效的大鼠肾移植静脉吻合方法.方法 建立同种异体肾移植动物模型.根据移植肾静脉重建方式不同进行模型分组:实验组(n=32):内支架法原位端一端吻合;对照组Ⅰ(n=25):原位端.端吻合;对照组Ⅱ(n=22):cuff管套扎法吻合;对照组Ⅲ(n=20):与受体下腔静脉端侧吻合.比较各组移植肾静脉手术操作时间、手术并发症发生率.结果 实验组供肾静脉切取用时同对照组Ⅲ间差异有统计学意义(9.08±0.88)main比(12.07±0.88)min,P<0.05];修整用时与对照组Ⅱ间差异有统计学意义(4.97±0.58)win比(8.14±0.64)min,P<0.05];对照组Ⅱ术后静脉血栓发生率最高,同实验组间差异有统计学意义(X2=4.77,P<0.05);实验组静脉吻合口狭窄的发生率最低,同对照组Ⅰ间差异有统计学意义(x2=3.90,P<0.05).结论 应用内支架法行移植肾静脉端.端吻合操作时间短、并发症少,可有效解决大鼠肾移植静脉重建的问题.

A method for end-to-end anastomosis of veins of the renal allograft in rats with endoprostheses

Objective To establish a new venous anastomosis technique for renal transplantation in rats. Methods By using 8-10 week-old male SD rats as both donors and recipients, allograft kidney transplantation animal model was established. Ninety-nine models were divided into 4 groups according to the ways of venous anastomosis. Thirty-two allografts' veins were rebuilt end-to-end in situ by way of endoprostheses (experimental group). The allografts' veins were anastomosed end-to-end in situ directly as control group Ⅰ (n=25). The allografts' veins of control group Ⅱ ( n = 22) were loop ligatured with cuff tubes before anastomosis. In the remaining 20 cases (control group Ⅲ ) ,the allografts' veins were anastomosed with recipients' inferior vena end-to-side. The operating time and complications of allografts' veins were observed. Results There was significant difference in time for harvesting donors' veins between ex-perimental groups and control group Ⅲ (9.08±0.88) rain vs (12.07±0.88) rain, P<0.05]. Compared with experimental group, the time for veins clipping in control group Ⅱ was prolonged significantly (4.97±0.58 ) rain vs (8.14±0.64) min, P<0, 05]. The incidence of phzbothrombosis was highest in control group Ⅱ than in experimental group (X2 = 4.77,P<0.05). There was significant difference in incidence of anastomotie stenosis between experimental group and control group Ⅰ(X2 =3.90, P<0.05). Conclusion To rebuild veins of transplanted kidney end-to-end in situ with endoprostheses can both save the operating time and decrease the incidence of complications.