| 栀子全时段融合指纹图谱的构建及特征峰的鉴定 |
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| 引用本文： | 王伟,付娟,万琴,李家春,黄文哲,王振中,萧伟.栀子全时段融合指纹图谱的构建及特征峰的鉴定[J].中草药,2018,49(9):2158-2162. |
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| 作者姓名： | 王伟 付娟 万琴 李家春 黄文哲 王振中 萧伟 |
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| 作者单位： | 江苏康缘药业股份有限公司;中药制药过程新技术国家重点实验室 |
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| 基金项目： | 重大新药创制项目科技重大专项“现代中药创新集群与数字制药技术平台”（2013ZX09402203） |
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| 摘 要： | 目的建立栀子的指纹图谱并采用电喷雾离子源-四极杆-飞行时间质谱(ESI-Q-TOF MS)对其共有峰进行定性分析。方法采用Kromasil 100-5 C18柱,以乙腈-0.1%磷酸梯度洗脱,体积流量为1 m L/min,柱温为35℃,检测波长为238、327、440 nm。使用Matlab 7.1软件编程,对3波长下的CSV格式数据进行全时段融合。结果各色谱峰分离度良好,3波长融合指纹图谱全面体现了栀子238、327、440 nm特征吸收波长的指纹信息,标定20个共有峰,并完成其中16个成分的鉴定。10批不同批次的栀子指纹图谱与对照指纹图谱之间相似度较好,相似度大于0.90以上。结论实现了栀子融合指纹图谱的测定,同时解析了图谱中的化学组成,并制定了主成分与栀子苷相对峰面积的范围,为全面控制药材质量提供了数据支撑。
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| 关 键 词： | 栀子 全时段融合指纹图谱 山栀苷 京尼平苷酸 去乙酰车叶草酸甲酯 京尼平龙胆双糖苷 栀子苷 新绿原酸 绿原酸 隐绿原酸 异绿原酸A 西红花苷-I 西红花苷-II |
| 收稿时间： | 2017/11/23 0:00:00 |
Study on all-time multi-wavelength fusion fingerprints of chemical components in Gardeniae Fructus and quantitative analysis of characteristic components |
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| WANG Wei,FU Juan,WAN Qin,LI Jia-chun,HUANG Wen-zhe,WANG Zhen-zhong and XIAO Wei.Study on all-time multi-wavelength fusion fingerprints of chemical components in Gardeniae Fructus and quantitative analysis of characteristic components[J].Chinese Traditional and Herbal Drugs,2018,49(9):2158-2162. |
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| Authors: | WANG Wei FU Juan WAN Qin LI Jia-chun HUANG Wen-zhe WANG Zhen-zhong and XIAO Wei |
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| Institution: | Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China,Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China,Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China,Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China,Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China,Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China and Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China |
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| Abstract: | |
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| Keywords: | Gardeniae Fructus all-time multi-wavelength fusion fingerprint shanzhiside geniposidic acid deacetylasperulosidic acid methyl ester genipin-1-D-gentiobioside geniposide neochlorogenic acid chlorogenic acid cryptochlorogenic acid isochlorogenic acid A crocin-I crocin-II |
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