基于SRAP分子标记技术研究浙江产细叶石仙桃的遗传多样性

目的以药用植物细叶石仙桃为材料,利用SRAP分子标记技术进行遗传多样性研究,为进一步开展该物种的保护奠定基础。方法从81对SRAP引物组合中,共筛选出7对用于SRAP-PCR,对浙江省境内5个自然居群的68份细叶石仙桃进行了遗传多样性分析。结果共获得197个电泳谱带,其中多态性条带为190条,每对引物平均提供27.29个标记信息,平均有效等位基因数目(N_e)为1.238 1。在物种水平上,多态位点百分率(PPB)为96.45%,N_ei’s基因多样性指数(H)为0.188 2,Shannon’s信息指数(I)为0.312 5;在居群水平上,PPB为30.46%~46.19%,平均为40%,H为0.089 1~0.155 9,平均0.120 8,I为0.138 7~0.234 9,平均0.186 4;5个居群的基因分化系数(G_(st))为35.81%,基因流(N_m)为0.896 3。UPGMA聚类结果表明,68份样品可分成5组,同一居群的细叶石仙桃聚于同一分支。结论细叶石仙桃具有较高的遗传多样性水平,居群间也存在一定的遗传分化和基因交流,但大部分遗传变异存在于居群内。

Genetic diversity analysis of Pholidota cantonensis in Zhejiang based on SRAP molecular marker technique

Objective To study the genetic diversity of medicinal plant Pholidota cantonensis Rolfe. by SRAP molecular marker technique, and to lay foundations for the protection of this species. Methods Seven pairs of primers were screened from 81 SRAP primer pairs, and used for SRAP-PCR; The genetic diversity of 68 P. cantonensis materials from five natural populations in Zhejiang Province was applied to analyze. Results Totally 197 reproducible bands were obtained, and 190 bands of which were polymorphic bands. Each primer pairs contributed 27.29 band on average, and the effective number of allele (N_e) was 1.238 1. At the species level, the percentage of polymorphic bands (PPB) was 96.45%, and the Nei''s genetic diversity (H) and the Shannon''s information index (I) were 0.188 2 and 0.312 5, respectively. At the population level, the PPB ranged from 30.46% to 46.19% with an average PPB of 40%, their H and I were 0.089 1-0.155 9 and 0.138 7 – 0.234 9, with average levels from 0.120 8 to 0.186 4, respectively. The coefficient of gene differentiation (G_st) for five populations was 35.81%, and the gene flow (N_m) was 0.896 3. The Unweighted Pair Group Method with Arithmetic (UPGMA) mean clustering results indicated that 68 samples were divided into five groups, and P. cantonensis plants from the same population were clustered into the same clade. Conclusion P. cantonensis showed a high level of genetic diversity, and its genetic differentiation as well as gene flow were found among the five populations; However, but the genetic differentiation happened mainly within each population.