| 西藏木瓜HPLC指纹图谱研究及多成分测定 |
| |
| 引用本文： | 赵志国,井凤,张敏敏,刘伟,李圣波,耿岩玲,王晓,赵恒强. 西藏木瓜HPLC指纹图谱研究及多成分测定[J]. 中草药, 2018, 49(10): 2454-2459 |
| |
| 作者姓名： | 赵志国 井凤 张敏敏 刘伟 李圣波 耿岩玲 王晓 赵恒强 |
| |
| 作者单位： | 齐鲁工业大学(山东省科学院)山东省分析测试中心山东省中药质量控制技术重点实验室;山东亚特生态技术股份有限公司 |
| |
| 基金项目： | 山东省自然科学基金资助项目（ZR2013HL029，ZR2017LH071）；山东省科技发展计划（2016GSF202014）；山东省泰山学者岗位资助项目；山东省科学院先导专项 |
| |
| 摘 要： | 目的建立西藏木瓜的HPLC多指标定量指纹图谱,为西藏木瓜的质量控制和真伪鉴别提供方法和技术支持。方法采用高剪切分散乳化(HSDE)法提取木瓜鲜样,采用Agilent Zorbax SB-C18(250 mm×4.6 mm,5.0μm)色谱柱,乙腈-0.5%冰乙酸+10 mmol/L乙酸铵水溶液为流动相,进行梯度洗脱,体积流量为0.8 m L/min,柱温为25℃,检测波长为280 nm。利用ESI-TOF/MS法对西藏木瓜提取物中多种化合物进行鉴别,在对多指标活性成分进行定量测定的基础上,建立西藏木瓜的HPLC指纹图谱。结果西藏木瓜HPLC指纹图谱共有30个共有峰,指认了其中9个峰,分别为绿原酸、香草酸、咖啡酸、藜芦酸、芸香苷、槲皮苷、金丝桃苷、齐墩果酸和熊果酸,并对其进行了定量测定。指纹图谱中相似度分析表明,10批西藏木瓜的相似度较为接近,而西藏木瓜与其他品种木瓜的相似度差别较大。结论 HSDE-HPLC-DAD-ESI-TOF/MS法可以用于木瓜鲜样的快速提取和分析,在此基础上建立的指纹图谱结合相似度分析可用于其质量评价,为提高西藏木瓜的质量控制水平提供了方法和技术支持。
|
| 关 键 词： | 西藏木瓜 高剪切分散乳化技术 高效液相色谱 电喷雾飞行时间质谱 多指标定量指纹图谱 |
| 收稿时间： | 2017-11-04 |
Multi-components determination and fringerprints analysis by HPLC in Chaenomeles thibetica |
| |
| ZHAO Zhi-guo,JING Feng,ZHANG Min-min,LIU Wei,LI Sheng-bo,GENG Yan-ling,WANG Xiao and ZHAO Heng-qiang. Multi-components determination and fringerprints analysis by HPLC in Chaenomeles thibetica[J]. Chinese Traditional and Herbal Drugs, 2018, 49(10): 2454-2459 |
| |
| Authors: | ZHAO Zhi-guo JING Feng ZHANG Min-min LIU Wei LI Sheng-bo GENG Yan-ling WANG Xiao ZHAO Heng-qiang |
| |
| Affiliation: | Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China,Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China,Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China,Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China,Shandong Yate Ecological Technology Co., Ltd., Linyi 266071, China,Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China,Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China and Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China |
| |
| Abstract: | |
| |
| Keywords: | Chaenomeles thibetica Yu HSDE HPLC ESI-TOF/MS multi-index quantitative fingerprint |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中草药》浏览原始摘要信息 |
|
点击此处可从《中草药》下载全文 |
|
