| 芪参益气方对H_2O_2损伤的心肌细胞线粒体结构和功能的保护作用及机制探讨 |
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| 引用本文： | 于佳慧,刘昕彦,黄凯,樊官伟,朱彦. 芪参益气方对H_2O_2损伤的心肌细胞线粒体结构和功能的保护作用及机制探讨[J]. 中草药, 2018, 49(14): 3343-3349 |
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| 作者姓名： | 于佳慧 刘昕彦 黄凯 樊官伟 朱彦 |
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| 作者单位： | 天津中医药大学天津市现代中药重点实验室;天津国际生物医药联合研究院中药新药研发中心;天津中医药大学第一附属医院 |
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| 基金项目： | 国家自然科学基金面上项目（81274128）；天津市高等学校创新团队（TD12-5031） |
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| 摘 要： | 目的制备H_2O_2诱导的心肌H9c2细胞损伤模型,通过线粒体结构、功能改变的多指标综合评价方法探讨芪参益气方对H9c2细胞的保护作用及作用机制。方法体外培养H9c2细胞,分为对照组、模型组(H_2O_2)、模型阳性对照羰基-氰-对-三氟甲氧基苯腙组(5μmol/L)、芪参益气方组(0.2 mg/mL),每组3个复孔,加入相应药物孵育24 h,H_2O_2诱导2 h制备心肌细胞损伤模型。采用Operetta高内涵成像系统评价H9c2细胞线粒体功能及形态纹理的改变;海马生物能量测定仪检测线粒体能量代谢的变化;流式细胞仪检测细胞的凋亡情况。结果芪参益气方能够明显降低H_2O_2损伤的H9c2细胞线粒体质量,增加线粒体膜电位和纹理的量化数值,同时增加线粒体基础耗氧率、ATP相关耗氧率、最大耗氧率以及储备能力,明显降低细胞早期及晚期凋亡率,增加活细胞率。结论基于细胞的线粒体结构和功能评价,证实芪参益气方通过改善线粒体功能及能量代谢减轻细胞凋亡保护H9c2细胞。
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| 关 键 词： | 芪参益气方 线粒体功能 细胞凋亡 H2O2损伤 线粒体膜电位 |
| 收稿时间： | 2018-02-02 |
Protective effect and mechanism of Qishen Yiqi Formula on mitochondrial structure and function of cardiomyocytes injured by H2O2 |
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| YU Jia-hui,LIU Xin-yan,HUANG Kai,FAN Guan-wei and ZHU Yan. Protective effect and mechanism of Qishen Yiqi Formula on mitochondrial structure and function of cardiomyocytes injured by H2O2[J]. Chinese Traditional and Herbal Drugs, 2018, 49(14): 3343-3349 |
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| Authors: | YU Jia-hui LIU Xin-yan HUANG Kai FAN Guan-wei ZHU Yan |
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| Affiliation: | Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin International Joint Academy of Biomedicine, Tianjin 300457, China,Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin International Joint Academy of Biomedicine, Tianjin 300457, China,Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin International Joint Academy of Biomedicine, Tianjin 300457, China,Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China and Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Tianjin International Joint Academy of Biomedicine, Tianjin 300457, China |
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| Abstract: | |
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| Keywords: | Qishen Yiqi Formula mitochondrial function apoptosis H2O2 injury mitochondrial membrane potential |
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