| 中药小分子单克隆抗体制备技术研究进展 |
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| 引用本文： | 章飚,李彦川,高雯,李细芬,张兰兰,李萍.中药小分子单克隆抗体制备技术研究进展[J].中草药,2018,49(10):2469-2476. |
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| 作者姓名： | 章飚 李彦川 高雯 李细芬 张兰兰 李萍 |
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| 作者单位： | 中国药科大学天然药物活性组分与药效国家重点实验室;数字本草中医药检测有限公司 |
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| 基金项目： | 天津市科技计划项目（17YFCZZC00350） |
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| 摘 要： | 近年来免疫分析技术在中医药领域广泛应用,尤其是在中药质量控制、中药安全性评价、中药(复方)机制研究等方面发挥了重要作用,其中中药小分子单克隆抗体的制备是这些应用的前提和关键。主要从完全抗原制备、动物免疫、杂交瘤细胞株建立及单克隆抗体生产等方面进行综述,特别对近20年已报道且具有代表性的中药小分子抗原抗体品种进行梳理,为中药小分子单克隆抗体的研究及应用提供参考。
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| 关 键 词： | 中药小分子 单克隆抗体 完全抗原制备 动物免疫 抗原抗体品种 |
| 收稿时间： | 2017/11/19 0:00:00 |
Research progress on preparation of small molecular monoclonal antibodies in Chinese materia medica |
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| ZHANG Biao,LI Yan-chuan,GAO Wen,LI Xi-fen,ZHANG Lan-lan and LI Ping.Research progress on preparation of small molecular monoclonal antibodies in Chinese materia medica[J].Chinese Traditional and Herbal Drugs,2018,49(10):2469-2476. |
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| Authors: | ZHANG Biao LI Yan-chuan GAO Wen LI Xi-fen ZHANG Lan-lan and LI Ping |
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| Institution: | State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China;Digital Materia Medica Traditional Chinese Medicine Testing Co., Ltd., Baoding 071200, China,Digital Materia Medica Traditional Chinese Medicine Testing Co., Ltd., Baoding 071200, China,State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China,Digital Materia Medica Traditional Chinese Medicine Testing Co., Ltd., Baoding 071200, China,Digital Materia Medica Traditional Chinese Medicine Testing Co., Ltd., Baoding 071200, China and State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China |
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| Abstract: | In recent years, immunoassay has been widely used in traditional Chinese medicine. It plays an important role in the quality control, the safety evaluation, and the mechanism research of Chinese medicine or compound medicine. Among them, the premise and key of the application is the preparation of monoclonal antibodies of small moleculars. This review summarized preparation of complete antigen, animal immunization, establishment of hybridoma cell lines, and production of monoclonal antibody. Especially, the varieties of representative antigen and antibody reported in recent 20 years were combed, which will provide the reference for the research and application of small molecular monoclonal antibodies of Chinese materia medica. |
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| Keywords: | small molecular in Chinese materia medica monoclonal antibody complete antigen preparation animal immunization variety of antigen and antibody |
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