| 云贵地区金铁锁EST-SSR遗传多样性分析 |
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| 引用本文： | 邱芬,雷瀚,陈杰,杨生超,白斌,刘成,叶鹏,高丽云,辛培尧. 云贵地区金铁锁EST-SSR遗传多样性分析[J]. 中草药, 2018, 49(16): 3895-3906 |
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| 作者姓名： | 邱芬 雷瀚 陈杰 杨生超 白斌 刘成 叶鹏 高丽云 辛培尧 |
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| 作者单位： | 西南林业大学西南山地森林资源保育与利用教育部重点实验室;毕节市中药研究所;云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心 |
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| 基金项目： | 云南省教育厅科学研究基金项目（2016ZZX52）；贵州省科技计划项目（黔科合SY字[2015]3027）；西南林业大学科研基金项目（XL21611） |
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| 摘 要： | 目的分析云贵地区金铁锁Psammosilene tunicoides遗传多样性和变异情况。方法采用转录组测序技术开发金铁锁的EST-SSR引物,并利用筛选出的分子标记对金铁锁进行多态性分析。结果共获得了17 530条含SSR位点的EST序列;筛选出了14对具有较高多态性的EST-SSR引物,对云贵地区的17份金铁锁进行多样性分析,发现其在位点水平上多态性信息含量(PIC)范围为0.350 0~0.795 0,具有较高的多态性;在群体水平上明显偏低,多态位点百分率(PPB)为64.29%~100%,Nei’s基因多样性指数的范围为0.188 2~0.477 7,平均0.323 2;云贵地区金铁锁群体内基因流(Nm)较小(Nm均值为0.302 0),群体间存在较大的遗传分化(Fst均值为0.452 9)。结论转录组测序丰富了金铁锁EST数据库;云贵地区金铁锁的遗传多样性可能与其繁殖方式、分布区较长的进化历史有关,群体间遗传分化大可能是地理阻隔截断了不同群体间的基因交流。
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| 关 键 词： | 金铁锁 云贵地区 EST-SSR 遗传多样性 多态性信息含量 |
| 收稿时间： | 2018-02-06 |
EST-SSR analysis of genetic diversity of Psammosilene tunicoides in Yunnan-Guizhou provincial region |
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| QIU Fen,LEI Han,CHEN Jie,YANG Sheng-chao,BAI Bin,LIU Cheng,YE Peng,GAO Li-yun and XIN Pei-yao. EST-SSR analysis of genetic diversity of Psammosilene tunicoides in Yunnan-Guizhou provincial region[J]. Chinese Traditional and Herbal Drugs, 2018, 49(16): 3895-3906 |
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| Authors: | QIU Fen LEI Han CHEN Jie YANG Sheng-chao BAI Bin LIU Cheng YE Peng GAO Li-yun XIN Pei-yao |
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| Affiliation: | Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China,Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China,Institute of Traditional Chinese Medicine of Bijie, Bijie 551700, China,National-Local Joint Engineering Research Center on Gemplasm Innovation & amp;Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming 650201, China,Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China,Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China,Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China,Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China and Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China |
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| Abstract: | |
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| Keywords: | Psammosilene tunicoides W.C.Wu et C.Y.Wu Yunnan-Guizhou region EST-SSR genetic diversity polymorphic information content |
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