| 响应面法优选葛根超微粉的制备工艺及活性成分测定研究 |
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| 引用本文： | 孔馨逸,刘妍如,唐志书,王梅,吕杨,张遥遥,董娇,宋忠兴,钱大玮.响应面法优选葛根超微粉的制备工艺及活性成分测定研究[J].中草药,2018,49(13):2993-3000. |
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| 作者姓名： | 孔馨逸 刘妍如 唐志书 王梅 吕杨 张遥遥 董娇 宋忠兴 钱大玮 |
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| 作者单位： | 陕西中医药大学陕西省中药资源产业化协同创新中心陕西省创新药物研究中心;陕西中医药大学附属医院;南京中医药大学药学院江苏省方剂高技术研究重点实验室江苏省中药资源产业化过程协同创新中心 |
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| 基金项目： | 国家自然科学基金资助项目（81501229）；陕西省科技厅“科技新星”培养计划（2017KJXX-71）；陕西省科技统筹创新工程计划项目（2015KTZDSF02-05-02）；陕西省创新药物研究中心（2017YWZX-01） |
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| 摘 要： | 目的建立葛根中8种主要黄酮类成分UPLC同时测定法,研究超微粉碎技术对葛根黄酮类成分含量的影响,确定葛根超微粉的生产工艺。方法采用响应面设计方法对葛根粉碎技术中的粉碎时间、投药量和初始粒度3个因素进行考察,以粒径分布(D90、D50)和UPLC法测定的不同粒径葛根粉(10~40、40~65、300目)中8个黄酮类活性成分(3′-羟基葛根素、葛根素、3′-甲氧基葛根素、大豆苷、染料木苷、大豆苷元、染料木素和芒柄花素)的含量数据,共10个指标作为响应因子进行葛根超微粉碎技术评价研究。结果葛根中8种黄酮类化合物分别在75.8~242.7、205.6~658.0、147.3~417.3、10.2~163.3、11.3~182.0、1.2~18.8、0.25~4.00、0.35~5.37μg/mL与色谱峰峰面积呈良好的线性关系,平均回收率为98.86%、99.25%、99.90%、100.17%、100.21%、101.40%、100.73%、101.42%,RSD均小于3.0%,UPLC同时测定结果显示,相较其他粒径葛根粉,葛根超微粉(300目)黄酮类含量更高。响应面法优选结果显示超微粉的最佳工艺参数条件为在粒径为80目(180.0±7.6)μm],通过5号筛时,投药量为247 g,粉碎时间为26 min。结论响应面法优选的葛根超微粉碎工艺简便,准确度较高,得到的超微粉黄酮含量更高。该工艺可以为葛根超微粉碎技术提供参考。
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| 关 键 词： | 葛根 超微粉 3''-羟基葛根素 葛根素 3''-甲氧基葛根素 大豆苷 染料木苷 大豆苷元 染料木素 芒柄花素 响应面法 超高效液相色谱法 |
| 收稿时间： | 2018/1/9 0:00:00 |
Optimization of ultrafine powders preparation process from Puerariae Lobatae Radix by response surface method and multiple isoflavonoids determination |
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| KONG Xin-yi,LIU Yan-ru,TANG Zhi-shu,WANG Mei,LV Yang,ZHANG Yao-yao,DONG Jiao,SONG Zhong-xing and QIAN Da-wei.Optimization of ultrafine powders preparation process from Puerariae Lobatae Radix by response surface method and multiple isoflavonoids determination[J].Chinese Traditional and Herbal Drugs,2018,49(13):2993-3000. |
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| Authors: | KONG Xin-yi LIU Yan-ru TANG Zhi-shu WANG Mei LV Yang ZHANG Yao-yao DONG Jiao SONG Zhong-xing and QIAN Da-wei |
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| Institution: | Shaanxi Province Drug Discovery Center of Innovation, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, China,Shaanxi Province Drug Discovery Center of Innovation, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, China,Shaanxi Province Drug Discovery Center of Innovation, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, China,The Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China,Shaanxi Province Drug Discovery Center of Innovation, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, China,Shaanxi Province Drug Discovery Center of Innovation, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, China,Shaanxi Province Drug Discovery Center of Innovation, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, China,Shaanxi Province Drug Discovery Center of Innovation, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, China and Collaborative Innovation Center of Chinese Medicinal Resources Industrialization of Jiangsu Province, Jiangsu Key Laboratory for Traditional Chinese Medical Formula Research, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| Abstract: | |
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| Keywords: | Puerariae Lobatae Radix ultrafine powder 3''-hydroxy puerarin puerarin 3''-methoxy puerarin daidzin genistin daidzein genistein formononetin response surface methodology UPLC |
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