ClC-3氯通道过表达对小鼠甲状腺结构及功能的影响

目的:研究过表达Cl C-3基因对小鼠甲状腺结构及功能的影响。方法:以3月龄FVB小鼠为实验对象,研究野生型(WT)小鼠和Cl C-3转基因小鼠甲状腺结构及功能的差异。用q PCR、Westren blot和免疫荧光技术观察小鼠甲状腺Cl C-3的表达与分布;对小鼠日常活动进行行为学监测;ELISA检测小鼠血清总三碘甲状腺原氨酸(TT3)、总甲状腺素(TT4)和促甲状腺激素(TSH)的浓度。结果:与WT组小鼠相比:Cl C-3转基因组小鼠甲状腺Cl C-3表达明显增多(P0.05);甲状腺表现为明显的增生,滤泡明显增大;体重减轻但摄食量增加(P0.05),毛发无光泽,行为活跃,易激惹;TT3、TT4明显增高(P0.05),但TSH变化不明显。结论:Cl C-3过表达可导致甲状腺组织增生,甲状腺激素分泌增多。本研究提示Cl C-3很可能参与了甲状腺激素的合成。

Effects of ClC-3 over-expression on structure and function of thyroid in mice

AIM: To study the effect of ClC-3 gene over-expression on thyroid structure and function in mice.METHODS: Three-months-old FVB mice were used to study the difference of thyroid structure and function between wild-type (WT) mouse and ClC-3 transgene mice. The expression and distribution of ClC-3 in the thyroid of mice were determined by the methods of qPCR, Western blot and immunofluorescence. Behavioral monitoring was performed on the daily activities of mice. Serum concentrations of total triiodothyronine (TT3), total thyroxine (TT4) and thyrotropin (TSH) were measured by ELISA.RESULTS: Compared with the WT group, the expression of ClC-3 in the thyroid of ClC-3 transgene group was significantly increased (P<0.05). The thyroid gland showed obvious hyperplasia and the folliculi glandulae thyreoideae was significantly bigger in ClC-3 transgene mice (P<0.05). The weight loss was increased in ClC-3 transgene mice (P<0.05). The expression of TT3 and TT4 were significantly higher than that of WT group (P<0.05), but the change of TSH was not obvious.CONCLUSION: ClC-3 over-expression results in thyroid hyperplasia and thyroid hormone secretion. This study suggests that ClC-3 is likely to be involved in the synthesis of thyroid hormones.