| HPLC-DAD法测定迷迭香茎和叶中11种抗氧化活性成分 |
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| 引用本文： | 许艺凡,刘普,刘佩佩,邱赛西,高晓丹,许肖锋,常盛,邓瑞雪.HPLC-DAD法测定迷迭香茎和叶中11种抗氧化活性成分[J].中草药,2018,49(9):2153-2157. |
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| 作者姓名： | 许艺凡 刘普 刘佩佩 邱赛西 高晓丹 许肖锋 常盛 邓瑞雪 |
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| 作者单位： | 河南科技大学化工与制药学院 |
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| 基金项目： | 国家自然科学基金资助项目（U1404831，U1604192）；河南科技大学大学生训练计划项目资助（2017125）；国家级大学生创新训练计划项目（201610464033） |
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| 摘 要： | 目的建立HPLC-DAD同时测定迷迭香叶和茎中11种抗氧化活性成分含量的方法。方法采用Zorbax SB-Aq C18色谱柱(250 mm×4.6 mm,5μm);以甲醇(A)-磷酸二氢钾缓冲盐(B)溶液为流动相,梯度洗脱,体积流量1.0 mL/min,柱温30℃,绿原酸、咖啡酸、迷迭香酸、木犀草素、芹菜素和芫花素的检测波长为328 nm,丹皮酚、迷迭香酚、橙皮素、鼠尾草酚和鼠尾草酸的检测波长为284 nm,进样量10μL。结果绿原酸、咖啡酸、迷迭香酸、丹皮酚、迷迭香酚、橙皮素、木犀草素、芹菜素、鼠尾草酚、芫花素和鼠尾草酸分别在2.02~64.64μmol/mL,r=0.999 97;1.74~55.68μmol/mL,r=0.999 97;2.76~88.32μmol/mL,r=0.999 97;0.24~7.68μmol/mL,r=0.996 63;0.46~14.72μmol/mL,r=0.999 97;0.22~7.04μmol/mL,r=0.998 64;0.72~23.04μmol/mL,r=0.999 37;3.74~119.68μmol/mL,r=0.999 80;5.1~163.28μmol/mL,r=0.996 18;0.4~12.8μmol/mL,r=0.996 35;5.22~167.04μmol/mL,r=0.996 98内有良好的线性关系;平均加样回收率为98.1%~100.5%,RSD为0.67%~2.14%。结论该方法准确,实用性好,可用于迷迭香叶和茎中抗氧化活性成分的定性与定量分析。
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| 关 键 词： | 迷迭香 高效液相色谱法 抗氧化成分 绿原酸 咖啡酸 迷迭香酸 木犀草素 芹菜素 芫花素 丹皮酚 迷迭香酚 橙皮素 鼠尾草酚 鼠尾草酸 |
| 收稿时间： | 2017/12/7 0:00:00 |
Simultaneous determination of main anti-oxidant constituents in Rosmarinus officinalis by HPLC-DAD |
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| XU Yi-fan,LIU Pu,LIU Pei-pei,QIU Sai-xi,GAO Xiao-dan,XU Xiao-feng,CHANG Sheng and DENG Rui-xue.Simultaneous determination of main anti-oxidant constituents in Rosmarinus officinalis by HPLC-DAD[J].Chinese Traditional and Herbal Drugs,2018,49(9):2153-2157. |
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| Authors: | XU Yi-fan LIU Pu LIU Pei-pei QIU Sai-xi GAO Xiao-dan XU Xiao-feng CHANG Sheng and DENG Rui-xue |
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| Institution: | School of Chemical Engineering & Pharmacy, Henan University of Science and Technology, Luoyang 471023, China,School of Chemical Engineering & Pharmacy, Henan University of Science and Technology, Luoyang 471023, China,School of Chemical Engineering & Pharmacy, Henan University of Science and Technology, Luoyang 471023, China,School of Chemical Engineering & Pharmacy, Henan University of Science and Technology, Luoyang 471023, China,School of Chemical Engineering & Pharmacy, Henan University of Science and Technology, Luoyang 471023, China,School of Chemical Engineering & Pharmacy, Henan University of Science and Technology, Luoyang 471023, China,School of Chemical Engineering & Pharmacy, Henan University of Science and Technology, Luoyang 471023, China and School of Chemical Engineering & Pharmacy, Henan University of Science and Technology, Luoyang 471023, China |
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| Abstract: | |
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| Keywords: | Rosmarinus officinalis L HPLC anti-oxidant constituents chlorogenic acid caffeic acid rosmarinic acid luteolin apigenin genkwanin paeonol rosmanol hesperetin carnosol carnosic acid |
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