基于代谢组学技术的黄芪根与地上部分醋酸乙酯部位的药效比较

目的比较黄芪根与地上部分醋酸乙酯部位的药效作用,为黄芪地上部分的资源利用提供依据。方法采用环磷酰胺复制白细胞减少症小鼠动物模型,以药效学指标与核磁共振(NMR)代谢组学相结合的方法对黄芪根和地上部分醋酸乙酯部位进行药效比较研究。结果黄芪根和地上部分醋酸乙酯部位均可升高白细胞数、单核细胞数、淋巴细胞数和中性粒细胞数;黄芪根醋酸乙酯部位可回调小鼠脾脏中异亮氨酸、亮氨酸、缬氨酸、精氨酸、肌酸、次黄嘌呤6个差异代谢物,黄芪地上部分醋酸乙酯部位可回调小鼠脾脏中精氨酸、天冬氨酸、肌酸、甘油磷酰胆碱、鲨肌醇5个差异代谢物,其治疗指数(EI)值分别为451.83和469.17。结论黄芪根与地上部分醋酸乙酯部位均具有较好的升高白细胞的作用,且升白机制均可能与能量代谢、氨基酸代谢、胆碱代谢和嘌呤代谢有关。

Efficacy comparative analysis between part of ethyl acetate in aerial parts and roots of Astragali Radix based on metabolomics

Objective To compare the efficacy between part of ethyl acetate in aerial parts and roots of Astragali Radix (AR), and to provide scientific evidences for the comprehensive utilization of aerial parts of Astragalus membranaceus (APAM).Methods An experimental mouse model of leucopenia was induced by cyclophosphamide, and efficacy indicators combined with NMR based on metabolomic approach were employed to compare the therapeutic effects of part of ethyl acetate in APAM and AR.Results Part of ethyl acetate in APAM and AR increased the contents of white blood cell (WBC), monocytes (MO), lymphocyte (LY), and neutrophils (NE). The levels of six potential endogenous metabolites (isoleucine, leucine, valine, arginine, creatine, and hypoxanthine) were reversed by part of ethyl acetate in APAM, and five potential endogenous metabolites (arginine, aspartate, creatine, GPC, and scyllo-Inositol) were reversed by part of ethyl acetate in AR. Efficacy indexes were 451.83 and 469.17.Conclusion Both parts of ethyl acetate in APAM and AR elevated WBC, the mechanisms of them on leukocytosis were probably related to energy metabolism, amino acids metabolism, choline metabolism, and purine metabolism.