肿瘤高甲基化基因1重要下游基因HMMR对乳腺癌细胞株MDA-MB-231迁移和侵袭能力影响的体外实验

Effect of hypermethylated in cancer 1-downstream-regulated gene HMMR on cell migration and invasion in breast cancer: an in vitro study on breast cancer cell line MDA-MB-231

Objective: To investigate the regulatory relationship between hypermethylated in cancer 1 (HIC-1) and hyaluronan-mediated motility receptor (HMMR) and to study the effect of HMMR on migration and invasion of MDA-MB-231 breast cancer cell strains. Methods: MDA-MB-231 cells were transfected with double-strand saRNA HIC-1 and double-strand siRNA HMMR, respectively. The expression of HIC-1 and HMMR in MDA-MB-231 cells were detected by RT-PCR and Western blotting. CCK-8 assay was used to detect cell proliferation after siRNA or saRNA transfection. Transwell assay was used to determine the effect of siRNA or saRNA transfection on migration and invasion of MDA-MB-231 breast cancer cells. Results: MDA-MB-231 breast cancer cells were successfully transfected. The up-regulation of HIC-1 induced HMMR down-regulation, and significantly inhibited cell migration and invasion of MDA-MB-231 breast cancer cells. HMMR was down-regulated by siRNA HMMR transfection leading to inhibition of cell migration and invasion. However, cell proliferation have not been affected in both saRNA HIC-1 and saRNA HMMR transfections. Conclusions: HMMR is regulated by HIC-1 and it plays an important role in cell migration and invasion. Inhibition of HIC-1 upregulates HMMR, thus promotes cell migration and invasion of breast cancer cells.