| 尿感方抗尿道致病性大肠杆菌侵袭膀胱上皮细胞的作用研究 |
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| 引用本文： | 吴雨,蒋健,贺敏,张磊阳,陈文文. 尿感方抗尿道致病性大肠杆菌侵袭膀胱上皮细胞的作用研究[J]. 中草药, 2018, 49(9): 2084-2089 |
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| 作者姓名： | 吴雨 蒋健 贺敏 张磊阳 陈文文 |
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| 作者单位： | 上海中医药大学附属曙光医院临床药理科 |
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| 基金项目： | 国家自然科学基金资助项目（81403168） |
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| 摘 要： | 目的观察尿感方抗尿道致病性大肠杆菌(uropathogenic Escheriehia coli,UPEC)侵袭膀胱上皮细胞的作用,并探讨其作用机制。方法通过UPEC感染人膀胱癌细胞5637(human bladder cancer cell 5637,HTB-9)侵袭模型,观察尿感方含药尿液保护膀胱上皮细胞抗UPEC侵袭的作用;同时采用分子生物学技术,观察其对Toll样受体4(TLR4)/环磷酸腺苷(c AMP)信号传导通路的主要环节:TLR4、腺苷酸环化酶3(AC3)、环磷酸腺苷(c AMP)、蛋白激酶A(PKA)和Rac-1的影响。结果与大鼠空白尿液高剂量组(体积分数10%空白尿液)比较,尿感方大鼠含药尿液高剂量组(体积分数10%含药尿液)的细菌入侵率降低;与模型组比较,大鼠空白尿液组的细菌入侵率无显著性差异。与大鼠空白尿液高剂量组比较,尿感方大鼠含药尿液高剂量组增加了TLR4、AC3蛋白的表达及胞内c AMP的水平,促进了PKA活化,抑制了Rac-1的活性。结论尿感方具有一定的抗UPEC侵袭膀胱上皮细胞的作用,其作用机制与影响TLR4/c AMP信号传导通路的环节有关。
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| 关 键 词： | 尿感方 尿道致病性大肠杆菌 侵袭 人膀胱上皮细胞 TLR4/cAMP信号传导通路 |
| 收稿时间： | 2017-09-29 |
Niaogan Prescription protects bladder epithelial cells against uropathogenic Escherichia coli invasion |
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| WU Yu,JIANG Jian,HE Min,ZHANG Lei-yang and CHEN Wen-wen. Niaogan Prescription protects bladder epithelial cells against uropathogenic Escherichia coli invasion[J]. Chinese Traditional and Herbal Drugs, 2018, 49(9): 2084-2089 |
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| Authors: | WU Yu JIANG Jian HE Min ZHANG Lei-yang CHEN Wen-wen |
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| Affiliation: | Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China,Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China,Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China,Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China and Department of Clinical Pharmacology, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai 201203, China |
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