| 牛樟芝非还原型聚酮合酶基因的克隆及表达分析 |
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| 引用本文： | 原晓龙,华梅,陈剑,陈中华,王娟,杨宇明,王毅. 牛樟芝非还原型聚酮合酶基因的克隆及表达分析[J]. 中草药, 2018, 49(20): 4870-4876 |
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| 作者姓名： | 原晓龙 华梅 陈剑 陈中华 王娟 杨宇明 王毅 |
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| 作者单位： | 云南省林业科学院;云南省森林植物培育与开发利用重点实验室;国家林业局云南珍稀濒特森林植物保护和繁育重点实验室 |
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| 基金项目： | 国家自然科学青年科学基金项目（31400488）；云南省面上基金资助项目（2016FB055）；云南省对外科技合作计划（2015IA004） |
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| 摘 要： | 目的获得牛樟芝聚酮合酶基因(Ac PKS1)全长,对其进行生物学分析并分析该基因在不同培养基上的表达差异。方法通过对牛樟芝基因组分析获得牛樟芝聚酮合酶基因,通过设计含有起始密码子和终止密码子的特异引物并以牛樟芝c DNA为模板克隆得到Ac PKS1基因全长,并对该基因进行生物信息学分析及在不同培养基上的表达谱分析。结果 Ac PKS1全长6 348 bp,含有6个内含子和7个外显子,外显子编码2 115个氨基酸;通过生物信息学分析,推测该基因为真菌I型非还原型PKS,结构域为SAT-KS-PT-ACP-ACP-TE,系统树分析显示Ac PKS1与其他未知功能的聚酮合酶(PKS)聚为一支,说明Ac PKS1可能是一种新的聚酮化合物环化方式;表达谱分析表明,葡萄糖为Ac PKS1基因表达的必要条件,且葡萄糖含量与Ac PKS基因表达量呈正相关。结论本研究为Ac PKS1功能鉴定以及牛樟芝基因资源利用奠定基础。
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| 关 键 词： | 牛樟芝 聚酮合酶 生物信息学分析 表达谱 AcPKS1 |
| 收稿时间： | 2018-03-06 |
Cloning and expressing of a polyketide synthase gene in Antrodia camphorata |
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| YUAN Xiao-long,HUA Mei,CHEN Jian,CHEN Zhong-hu,WANG Juan,YANG Yu-ming and WANG Yi. Cloning and expressing of a polyketide synthase gene in Antrodia camphorata[J]. Chinese Traditional and Herbal Drugs, 2018, 49(20): 4870-4876 |
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| Authors: | YUAN Xiao-long HUA Mei CHEN Jian CHEN Zhong-hu WANG Juan YANG Yu-ming WANG Yi |
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| Affiliation: | Yunnan Academy of Forestry, Kunming 650204, China;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry, Kunming 650204, China;Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the State Forestry Administration, Kunming 650204, China,Yunnan Academy of Forestry, Kunming 650204, China;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry, Kunming 650204, China;Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the State Forestry Administration, Kunming 650204, China,Yunnan Academy of Forestry, Kunming 650204, China;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry, Kunming 650204, China;Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the State Forestry Administration, Kunming 650204, China,Yunnan Academy of Forestry, Kunming 650204, China;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry, Kunming 650204, China;Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the State Forestry Administration, Kunming 650204, China,Yunnan Academy of Forestry, Kunming 650204, China;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry, Kunming 650204, China;Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the State Forestry Administration, Kunming 650204, China,Yunnan Academy of Forestry, Kunming 650204, China;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry, Kunming 650204, China;Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the State Forestry Administration, Kunming 650204, China and Yunnan Academy of Forestry, Kunming 650204, China;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants, Yunnan Academy of Forestry, Kunming 650204, China;Conservation of Rare, Endangered & Endemic Forest Plants, Public Key Laboratory of the State Forestry Administration, Kunming 650204, China |
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| Abstract: | |
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| Keywords: | Antrodia camphorate M. Zang & C. H. Su polyketides synthases bioinformatics expression profiles AcPKS1 |
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