| 欧前胡素通过Nrf2/HO-1抗氧化途径对哮喘模型小鼠气道炎症的影响 |
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| 引用本文： | 咸哲民,姜京植,赵雨喆,王重阳,李俊峰,朴玉华,延光海,李良昌,朴红梅.欧前胡素通过Nrf2/HO-1抗氧化途径对哮喘模型小鼠气道炎症的影响[J].中草药,2018,49(19):4596-4601. |
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| 作者姓名： | 咸哲民 姜京植 赵雨喆 王重阳 李俊峰 朴玉华 延光海 李良昌 朴红梅 |
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| 作者单位： | 延边大学附属医院呼吸内科;延边大学医学院解剖学教研室 |
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| 基金项目： | 国家自然科学基金资助项目（81660003，81560679） |
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| 摘 要： | 目的探究欧前胡素对哮喘模型小鼠气道炎症的影响及其作用机制。方法将50只雄性BALB/c小鼠随机分成5组,即对照组、模型组及欧前胡素低、中、高剂量(15、30、60 mg/kg)组。采用HE、Masson、PAS染色观察小鼠肺组织病理学改变;ELISA法检测小鼠肺泡灌洗液(BALF)中免疫球蛋白E(Ig E)及白细胞介素-4(IL-4)、IL-5、IL-13、γ-干扰素(IFN-γ)水平;双氢罗丹明(DHR)-123法检测小鼠BALF中活性氧(ROS)的含量;检测小鼠肺组织中蛋白羰基含量及丙二醛(MDA)水平;抗氧化酶试剂盒检测小鼠BALF中超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)水平及总抗氧化能力(TAOC);免疫组织化学法和Western blotting法检测小鼠肺组织中核因子E2相关因子2(Nrf2)及血红素氧合酶1(HO-1)蛋白表达水平。结果与模型组比较,欧前胡素能够减少哮喘小鼠肺组织炎症细胞的渗出、杯状细胞的增生和胶原沉积;减少BALF中ROS、总Ig E和卵清蛋白(OVA)特异性Ig E的表达;降低IL-4、IL-5、IL-13、蛋白羰基、MDA的量并增加IFN-γ、SOD、GSH水平及TAOC;提高肺组织中Nrf2和HO-1蛋白表达水平。结论欧前胡素对OVA诱导的哮喘模型小鼠具有治疗作用,其作用机制可能与Nrf2/HO-1通路活化有关。
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| 关 键 词： | 欧前胡素 哮喘 核因子E2相关因子2 血红素加氧酶1 抗氧化 |
| 收稿时间： | 2018/5/4 0:00:00 |
Effect of imperatorin on airway inflammation of asthmatic mice model by Nrf2/HO-1 anti-oxidant pathway |
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| XIAN Zhe-min,JIANG Jing-zhi,ZHAO Yu-zhe,WANG Chong-yang,LI Jun-feng,PIAO Yu-hu,YAN Guang-hai,LI Liang-chang and PIAO Hong-mei.Effect of imperatorin on airway inflammation of asthmatic mice model by Nrf2/HO-1 anti-oxidant pathway[J].Chinese Traditional and Herbal Drugs,2018,49(19):4596-4601. |
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| Authors: | XIAN Zhe-min JIANG Jing-zhi ZHAO Yu-zhe WANG Chong-yang LI Jun-feng PIAO Yu-hu YAN Guang-hai LI Liang-chang and PIAO Hong-mei |
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| Institution: | Department of Respiratory Medicine, Yanbian University Hospital, Yanji 133000, China,Department of Anatomy, School of Basic Medical Sciences, Yanbian University, Yanji 133002, China,Department of Respiratory Medicine, Yanbian University Hospital, Yanji 133000, China,Department of Anatomy, School of Basic Medical Sciences, Yanbian University, Yanji 133002, China,Department of Anatomy, School of Basic Medical Sciences, Yanbian University, Yanji 133002, China,Department of Respiratory Medicine, Yanbian University Hospital, Yanji 133000, China,Department of Anatomy, School of Basic Medical Sciences, Yanbian University, Yanji 133002, China,Department of Anatomy, School of Basic Medical Sciences, Yanbian University, Yanji 133002, China and Department of Respiratory Medicine, Yanbian University Hospital, Yanji 133000, China |
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| Abstract: | |
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| Keywords: | imperatorin asthma Nrf2 HO-1 anti-oxidant |
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