| 新型寡核苷酸对乳腺癌细胞葡萄糖神经酰胺合酶与P-糖蛋白表达的影响 |
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| 引用本文： | 殷冬梅,贾辛,朱蕙霞,沈勤. 新型寡核苷酸对乳腺癌细胞葡萄糖神经酰胺合酶与P-糖蛋白表达的影响[J]. 江苏大学学报(医学版), 2010, 20(6) |
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| 作者姓名： | 殷冬梅 贾辛 朱蕙霞 沈勤 |
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| 作者单位： | 南通大学医学院生物化学教研室,江苏,南通,226001 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的: 观察新型反义寡核苷酸MBO-asGCS对乳腺癌细胞中葡萄糖神经酰胺合酶(GCS) 与 P-糖蛋白(P-gp)表达的影响.方法: 实时PCR检测MBO-asGCS处理的耐药型乳腺癌细胞MCF-7-AdrR,蛋白质印迹分析MCF-7-AdrR及其转化细胞中GCS与P-gp的蛋白表达,TUNEL法检测MBO-asGCS处理的MCF-7-AdrR中的细胞凋亡情况.结果: 在耐药株和GCS过表达的MCF-7-AdrR/GCS中GCS和P-糖蛋白表达特征性上升,而在asGCS转化细胞MCF-7-AdrR/asGCS中GCS和P-糖蛋白表达明显下降; MBO-asGCS处理的MCF-7-AdrR中GCS基因表达下调63.6%,凋亡细胞显著增加(P<0.01).结论: 乳腺癌细胞的耐药机制中可能存在GCS和P-糖蛋白的某种关联,升高的GCS可促进神经酰胺的代谢并增强P-糖蛋白的表达,产生耐药现象,反义寡核苷酸可抑制GCS表达,下调P-糖蛋白并促进凋亡.
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| 关 键 词： | 葡萄糖神经酰胺合酶 P-糖蛋白 反义寡核苷酸 凋亡 |
Effect on expression of glucosylceramide synthase and P-glycoprotein under new oligonucleotide treatment in breast cancer cells |
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| YIN Dong-mei,JIA Xin,ZHU Hui-xia,SHEN Qin. Effect on expression of glucosylceramide synthase and P-glycoprotein under new oligonucleotide treatment in breast cancer cells[J]. Journal of Jiangsu University Medicine Edition, 2010, 20(6) |
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| Authors: | YIN Dong-mei JIA Xin ZHU Hui-xia SHEN Qin |
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| Affiliation: | YIN Dong-mei,JIA Xin,ZHU Hui-xia,SHEN Qin(Department of Biochemistry,Medical College,Nantong University,Nantong Jiangsu 226001,China) |
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| Abstract: | Objective: To investigate the effect on expression of glucosylceramide synthase(GCS) and P-glycoprotein in drug-resistant breast cancer cells under a new oligonucleotide(MBO-asGCS) treatment.Methods: GCS mRNA expression was analyzed by realtime-PCR on MCF-7-AdrR cell treated with MBO-asGCS,expression of GCS and P-glycoprotein were assessed by western-blot analysis,apoptosis cells were labeled by terminal deoxynucleotide transferase-mediated dUTP nick end labeling(TUNEL).Results: Both of GCS and P-glycoprote... |
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| Keywords: | glucosylceramide synthase P-glycoprotein antisense oligonucleotide apoptosis |
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