植入PHPMA水凝胶对脑损伤后胶质瘢痕形成的抑制性影响

人工合成 PHPMA水凝胶材料。利用此材料修补急性损伤的大鼠脑皮质缺损区 ,评价其对创伤后胶质瘢痕形成的影响以及在神经组织再生中的作用。合成的水凝胶材料经检测分析性状后植入预先造成的大脑皮质空腔内 ,4周以后灌注取脑 ,观察植入材料和周围组织整合结果以及星形胶质细胞在材料周围和内部的浸润状况 ,确定有无胶质瘢痕的形成 ,并进行超微结构分析。结果表明 ,植入材料可和周围组织良好整合 ,二者交界处细胞分布弥散 ,星形胶质细胞无过度聚集现象 ;植入材料内部有轴突样结构生长和血管发生。本研究结果提示 ,利用 PHPMA水凝胶填补脑损伤后的空洞可抑制胶质瘢痕的形成 ,有利于损伤部位神经组织的再生。

THE INHIBITORY INFLUENCE OF INTRACEREBRALLY TRANSPLANTED PHPMA HYDROGEL ON THE FORMATION OF GLIAL SCAR IN THE RAT

PHPMA hydrogel was synthesized artificially and used to repair the cavity caused by acute injury in the cortex of the rat in order to evaluate its influence on the formation of glial scar and the regeneration of the neural tissue. The porous hydrogel was obtained by heterophase separation. After being analyzed, the hydrogel was implanted into the injured cortex of rats. The brain from those rats was processed by histochemistry and immunohistochemistry to observe the integration of the material and the brain tissue, as well as the immigration and infiltration of astrocytes at 4 weeks after transplantation. The grafts were also observed under the electron microscope. The grafted hydrogel was integrated with normal tissue quite well. No phenomenon of astrocytes aggregation occured at the budge region. Some fibers and nascent vessels could be seen in the grafts. The implanted PHPMA hydrogel could inhibit the formation of glial scar in the lesion area of brain tissue at some extent and might be beneficial to the neural regeneration in the CNS.