| 5-氮胞苷体外诱导骨髓间质干细胞分化为心肌细胞的实验研究 |
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| 引用本文： | 吴铁军,汤服民,蔡振杰,俞世强,周和平.5-氮胞苷体外诱导骨髓间质干细胞分化为心肌细胞的实验研究[J].中国心血管病研究杂志,2006,4(1):60-61. |
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| 作者姓名： | 吴铁军 汤服民 蔡振杰 俞世强 周和平 |
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| 作者单位： | 平顶山152中心医院胸心外科，平顶山152中心医院胸心外科，第四军医大学西京医院心血管外科，第四军医大学西京医院心血管外科，第四军医大学西京医院心血管外科 |
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| 摘 要： | 目的观察5-氮胞苷对骨髓间质干细胞(BMMSC)体外向心肌细胞诱导分化的影响.方法分离大鼠BMMSC体外培养,使用终浓度为10 μmol/L的5-氮胞苷(5-aza)对其定向诱导,观察细胞形态学的变化,荧光免疫组织化学方法进行鉴定,电镜观察细胞的超微结构.结果BMMSC体外经5-aza诱导4周,肌钙蛋白及Connexin43表达阳性,电镜下在部分细胞内肌节样结构及细胞间形成缝隙连接.结论BMMSC可以在体外经5-氮胞苷诱导分化为心肌细胞.
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| 关 键 词： | 干细胞 细胞分化 阿扎胞苷 |
| 文章编号： | 1672-5301(2006)1-060-02 |
| 修稿时间： | 2005年5月19日 |
5-Azacytidine induces differentiation of bone marrow mesenchymal stem cells to cardiomyocytes |
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| WU Tie-jun,TANG Fu-min,CAI Zhen-Jie,et al..5-Azacytidine induces differentiation of bone marrow mesenchymal stem cells to cardiomyocytes[J].Chinese Journal of Cardiovascular Review,2006,4(1):60-61. |
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| Authors: | WU Tie-jun TANG Fu-min CAI Zhen-Jie |
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| Institution: | WU Tie-jun,TANG Fu-min,CAI Zhen-Jie,et al. Department of Cardiovascular Surgery,the Military 152 Hospital,Pingdingshan 467000,China |
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| Abstract: | Objective To study cardiac differentiation of bone marrow mesenchymal stem cell (BMMSC) induced by 5-azacytidine. Methods BMMSC from adult rats were treated with 5-aza for 24 h. After culture for 4 weeks, Differentiation was identified by immunochemistry technique and transmission electron microscopy. Results The expressions of Troponin I and Connexin43 at protein level were induced in 5-aza-treated BMMSC. Electron microscopy showed sarcomeric organization and gap junction in 5-aza-treated cells. Conclusion BMMSC could be induced to differentiate into cardiomyocytes by 5-aza. |
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| Keywords: | Bone marrow mesenchymal stem cells Cell differentiation Azacytidine |
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