微卫星DNA与生化标记分析对长爪沙鼠群体遗传分析的比较

目的比较生化标记和微卫星DNA标记方法对长爪沙鼠群体遗传分析的可靠性。方法应用27个生化位点和13个微卫星DNA位点,采用已建立的生化标记和微卫星DNA标记分析方法对国内2个长爪沙鼠群体进行遗传分析,计算并比较两种方法测得的各群体遗传参数。结果生化基因位点中有13个位点在整体中呈现遗传多态性,多态率为48.1%;微卫星位点中有11个位点在整体中表现出多态性,多态率均为84.6%。两种方法测得的平均有效等位基因数趋于一致,微卫星DNA的多态位点百分率和平均杂合度均明显高于生化标记方法。但生化标记和微卫星DNA检测对两个长爪沙鼠群体的遗传多样性差异反映一致,所反映的群体平衡状况也基本一致。结论生化标记分析和微卫星DNA方法均可较好地反映长爪沙鼠群体遗传结构。

Comparison of Population Genetic Analysis with Microsatellite DNA and Biochemical Markers in Mongolia Gerbils

Objective To compare the reliability of genetic analysis by biochemical markers and microsatellite DNA in Mongolia gerbil colonies.Method To analyze 27 biochemical marker loci and 13 microsatellite DNA loci of two Mongolia gerbil colonies and compare the results obtained by the two techniques.Result 13 biochemical marker loci of the 27 loci showed polymorphism in the whole population,and the polymorphism rate was 48.1%.11 microsatellite DNA loci were polymorphic in the whole population,and the polymorphism rate was 84.6%.The average effective allelic number derived from biochemical marker analysis was similar to that from microsatellite DNA analysis.Percentage of polymorphic loci and average homozygosity of microsatellite DNA were higher than those obtained by biochemistry marker analysis.But the results of biochemistry marker and microsatellite DNA analyses were coincident in genetic diversity and Hardy-Weinberg equilibrium.Conclusion Both biochemistry maker and microsatellite DNA marker analyses are good methods for analyzing the population genetics of Mongolia gerbil colonies.