| 四环素调控SV40Tag转基因小鼠模型的建立 |
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| 引用本文： | 孙强,岳挺,刘英,冯洁,董娟,曹慧,章蓉,朱洪,刘全海,李厚达. 四环素调控SV40Tag转基因小鼠模型的建立[J]. 实验动物与比较医学, 2006, 0(1): 8-11 |
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| 作者姓名： | 孙强 岳挺 刘英 冯洁 董娟 曹慧 章蓉 朱洪 刘全海 李厚达 |
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| 作者单位： | 扬州大学比较医学中心，扬州大学比较医学中心，上海市医药工业研究院药理室，扬州大学比较医学中心，扬州大学比较医学中心，扬州大学比较医学中心，扬州大学比较医学中心，扬州大学比较医学中心，上海市医药工业研究院药理室，扬州大学比较医学中心 扬州225009，上海市医药工业研究院药理室，上海200437，扬州225009，上海200437，扬州225009，扬州225009，扬州225009，扬州225009，扬州225009，上海200437，扬州225009 |
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| 摘 要： | 目的构建四环素调控的SV40T转基因小鼠模型。方法同时显微注射外源基因p205-rtTA-C3和pTRE-Tag至FVB小鼠原核,注射受精卵移植到同期发情的假孕受体出生个体,经PCR和Southern检测获得阳性转基因小鼠。结果经PCR结合Southern检测得到rtTA和Tag双阳性转基因小鼠一只,rtTA单阳性两只和Tag单阳性一只。结论通过饮水给与四环素的双阳性小鼠可在卵巢中检测到Tag mRNA的表达。
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| 关 键 词： | 四环素 基因表达调控系统 转基因 模型,动物 |
| 修稿时间： | 2004-11-05 |
Establishment of Tetracycline-Dependent Regulation of SV40 Tag Transgenic mice |
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| SUN Qiang,YUE Ting,LIU Ying,FENG Jie,DONG Juan,CAO Hui,ZHANG Rong,ZHU Hong,LIU Quan-hai,LI Hou-da. Establishment of Tetracycline-Dependent Regulation of SV40 Tag Transgenic mice[J]. Laboratory Animal and Comparative Medicine, 2006, 0(1): 8-11 |
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| Authors: | SUN Qiang YUE Ting LIU Ying FENG Jie DONG Juan CAO Hui ZHANG Rong ZHU Hong LIU Quan-hai LI Hou-da |
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| Abstract: | Objective To establish a tetracycline-dependent regulation of SV40 Tag transgenic mice.Methods p205-sv40Ta p205-rtTA-C3 and pTRE-Tag dual transgenic mice was generated by pro-nuclear microinjection and embryo transplantation.Positive founder mice were identified through both PCR and Southern blot.Results A rtTA and Tag dual positive transgenic mice,two rtTA single and one Tag single transgenic mice were confirmed by PCR and Southern Blot.The expression of Tag mRNA was detected by RT-PCR in the ovary of the dual transgenic mice which were administered with tetracycline in the drinking water.Conclusion The results of this study indicate that it is possible to establish a tetracycline-dependent regulation of SV40 Tag transgenic mice by administeration of tetracycline in the drinking water. |
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| Keywords: | Tetracycline Gene Expression Regulation Transgenic Models Animal |
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