SHIV病毒在猴体内的复制与传代

目的为建立SHIV艾滋病动物模型提供毒力较强的病毒株,将新合成的SHIV XJ02170病毒适应猴体,并增强其毒力。方法实验前采集猴血清并进行血清学检查和PCR检测。选出13只无SIV,STLV1,SRV D和B病毒感染的猴。第一批实验,将SHIV前病毒DNA质粒经肌肉注射到猴体内,每只500μg;SHIV病毒液,经静脉注射到猴体内,每只2ml。病毒质粒和病毒液各接种2只猴。当第一批猴体检出病毒后,10ml感染猴的全血,抗凝后静脉注射到第二批猴体内,当第二批猴检出病毒后再将10ml感染猴的全血静脉注射到第三批猴体内,连续传代4次。每批实验均定期采集血液标本,分别用肝素和EDTA抗凝,进行病毒分离;病毒基因PCR检测;CD4,CD8测定;病毒抗体检测。结果SHIV XJ02170病毒和SHIV XJ02170前病毒DNA质粒在猴体内的传代中均能分离出病毒;从传代猴的血浆和外周血单核细胞(PBMC)中检出了病毒DNA和RNA基因;CD4,CD8测定结果显示有暂时性倒置现象,后变为正常倒置与正常交替出现;在传代的猴血清中检测出特异性HIV病毒抗体。结论SHIV XJ02170病毒与SHIV XJ02170前病毒DNA质粒,均能在恒河猴体内复制。

Replication and Passage of SHIV Proviral DNA and Virus in Rhesus Monkeys

Objective To explore the sequential pathological changes during the process of establishing high fat diet fatty liver model in rats. Methods Sixty male Wistar rats (180-220 g) were divided randomly into six groups: normal control Groups (1,2,3) fed with common feed and model groups(4,5,6) fed with high fat feed (93.8% foundation feed,1% cholesterol,5% lard,0.2% bilesalt)for 4,8,12 weeks. Results The liver index of the model group 4 is significantly higher than those of group 5 , 6 and normal control groups;but the liver index of the model group 5 , 6 is significantly lower than which of the control groups. The histological observation shows that the form of lipid droplet of the liver cell is mainly grain type, small bubble, large bubble in early, medium and advanced stage with the area of the vacuole significantly increasing one by one .The ultrastructural observation on the liver of the model groups'' rats shows that the quantity of RER and SER is significantly different in each stage. Conclusion The lipid droplet form , area of vacuole ,quantity of RER and SER should be the evaluating indicator in the estimation of high fat diet fatty liver model, but the liver index and the variety of chondriosome lacks to be .