新生猪缺氧后心肌连接蛋白Cx43表达的变化

目的探讨新生猪缺氧后心肌连接蛋白Cx43 mRNA和蛋白含量的变化。方法对照组(C组):新生猪6头,未吸入低氧;缺氧组(H组):新生猪6头,吸入低氧(FiO2=0.1)维持1 h。于缺氧结束后5 h,免疫荧光染色检测心肌组织细胞缝隙连接蛋白Cx43蛋白的表达,实时荧光定量逆转录多聚酶链式反应(RT-PCR)检测心肌组织Cx43 mRNA的表达。结果H组心肌组织Cx43蛋白的表达显著低于C组(P<0.01),但两组心肌组织Cx43 mRNA表达差异无统计学意义(P>0.05)。结论缺氧可导致心肌Cx43蛋白的表达或分布发生变化。

Expression of Cx43 in the Myocardia of Hypoxic Newborn Piglets

Objective To discuss isolation and culture methods of primary rat skeletal muscle satellite cells and observe its characterization of proliferation and differentiation in vitro culture. Methods Primary muscle satellite cells of relative high purity were obtained by improved two-steps enzymatic digestion combined with pre-plating techniques. Marker of muscle-derived cell, desmin, was identified by histocytochemistry. The characterization of cell proliferation was studied by the way of MTT assay experiment. Differentiation of muscle satellite cell under different culture conditions in vitro was also studied. Results The purity of harvested muscle satellite cell was more than 90 percent. When cultured in vitro, the latent phase of the cell is at the 1st to 2nd days, platform phase is at the 5th to 6th day. It shows strong proliferative ability in vitro culture and could fuse with each other into myotube cells without special induction. Conclusions Muscle satellite cells by in vitro culture were enough to meet study of tissue engineering and cell transplantation.