VEGF反义寡核苷酸抑制小鼠Lewis肺癌生长的研究

目的探讨血管内皮生长因子(VEGF)反义寡核苷酸对C57BL/6小鼠Lewis肺癌肿瘤生长的抑制作用。方法制备C57BL/6小鼠皮下肺癌模型30只,随机分为3组,每组10只VEGF反义寡核苷酸(ASPODN)治疗组、VEGF正义寡核苷酸(SPODN)治疗组及对照组。接种Lewis肺癌细胞后24h内,分别皮下注射ASPODN及SPODN进行治疗,对照组只注射生理盐水,每周2次,连续4周;观察各组小鼠肿瘤的生长情况、游标卡尺测量肿瘤体积大小。所有C57BL/6小鼠于接种后第25天先用二维超声观察肿瘤的实时图象,利用脉冲多普勒获取血流频谱,获得收缩期峰值速度(PS),阻力指数(RI)。断颈处死小鼠,光镜及电镜下观察肿瘤组织形态学改变及超微结构变化,免疫组化法检测微血管密度(MVD)。结果与对照组瘤重比较(7.83±0.78)g、VEGF-ASPODN组(4.49±0.43)g能明显抑制小鼠肿瘤生长(P<0.01)、VEGF-SPODN组(7.73±0.69)g则无明显作用(P>0.05)。VEGF-ASPODN组、VEGF-SPODN组抑瘤率分别为42.7%、5.9%。组织形态学及超微结构观察,VEGF-ASPODN对肿瘤生长具有抑制作用。VEGF-ASPODN组与对照组相比较PS及RI有明显差异(P<0.01);VEGF-SPODN组与对照组相比无明显差异(P>0.05)。结论肿瘤原位注射VEGF反义寡核苷酸能抑制小鼠肺癌生长。

Inhibition of the Growth of Mouse Lewis Lung Cancer by VEGF Antisense Oligonucleotides

Objective To establish a breeding colony of SPF golden hamsters for improving quality of Japanese Encephalitis vaccine. Methods The golden hamsters were cleansed by cesarean operation and the baby golden hamsters were fostered in isolators by SPF golden hamsters from the Harlan in Germany. Results In the end of last year, there were more than 200 pairs of SPF golden hamsters in the isolators. The examination of the pathogens showed, the colony was upgraded to the standard of SPF golden hamsters. Conclusion The SPF golden hamsters breeding colony is established successfully by cesarean operation and have been mass-produced in the barrier.