大鼠自发性乳腺癌瘤株的建立和病理学研究

目的]评价重组人干扰素α2b鼻腔喷雾剂对预防治疗SARS-CoV病毒感染恒河猴的作用。方法]10只恒河猴随机分为2组,每组5只。分别为试验组(重组人干扰素α2b鼻腔喷雾剂)和对照组(空白干扰素)。分别在攻毒前19h和1h,攻毒后7h、23h、31h、47h、55h、71h、95h和119h不同时间经鼻吸入受试物,0.4ml/只。按计划定时取咽拭子做real-timePCR检测和病毒分离;取静脉血做病毒分离检测、中和抗体、IgG、血常规、血生化和血凝指标。结果]1.SARS感染对照组:全部动物咽拭子标本经real-timePCR均检出SARS-CoV病毒,持续时间从攻毒后2天至8天。攻毒后2天3只、5天1只、7天2只,其咽拭子标本中病毒分离阳性,进一步证实病毒在体内的复制。攻毒后,诱导产生高滴度的抗体和中和抗体,证实病毒感染。大体解剖全部动物肺脏为灰白色,有大面积出血,其中2只动物肺脏与胸壁有粘连,组织病理称典型SARS肺炎性病变。2.干扰素试验组:和对照组相同时间取的咽拭子等标本中,real-timePCR检测和病毒分离均未检出病毒。攻毒后病毒导致机体产生的中和抗体和IgG抗体的反应很弱。血常规、血生化和血凝指标结果表明干扰素试验组动物攻毒后各项指标较试验前比较没有显著性改变;大体解剖动物肺脏为灰粉色,4只动物肺脏有少量出血,其中1只动物肺脏与胸壁有粘连,组织病理未见典型SARS肺炎性病变。因而认为,重组人干扰素α2b鼻腔喷雾剂可以有效阻断SARS-CoV病毒对恒河猴的感染;;为预防人类感染SARS提供参考。

Establishment of Rat Spontaneous Mammary Adenocarcinoma Tumor Strain to Generate the Type C Oncovirus and its Pathological Studies

Objective] The present work is to establish rat spontaneous mammary adenocarcinoma tumor strain to produce the type C oncovirus and investigate the pathological characteristics of rat spontaneous mammary adenocarcinomas caused by type C oncovirus. Methods: The tumor strain of rat mammary adenocarcinomas was established by alternate descent generations of carcinoma struma and cell suspension. For pathological observation, rat mammary primary adenocarcinoma and implant adenocarcinoma were respectively fixed in 10% formaline, embedded in paraffins, routinely stained with HE and Ag. For electron microscope observation, rat mammary primary carcinoma and implant carcinoma were double fixed in 2.5% glutaraldehyde and 1% osmic acid respectively, embedded in Epon 812, ultrathin sectioned, double stained with uranium acetate and lead citrate, and observed under transmission electron microscope (TEM). Results] The volume of rat mammary primary carcinoma cells is quite small, only 10 (m in diameter. The typical tubular structures are found to be in such shapes as of globe, pappila, tortuousribbon and whorl. The carcinoma cells are separated into irregular nest structure by the reticular fibres after Ag staining. The above-mentioned shows that, as a type of rat mammary epithelial anaplastic adenocarcinomas, rat mammary carcinoma is originated from epithelial tissues. The implant carcinoma cells are round, cubic, and polygonal in shape, and carcinoma gigantic cells, pale cells and the like appeared, which are arranged in such structures as of nest, layer, trabecula, pappila, glomerate, adenocarcinoma tubule, and space, with heteomorphosis notable. The carcinoma cells are irregular in size, with large nuclei and increased nucleolus. Besides, there are bundles of tension protofibril in cytoplasm and seldom seen secretory granules. Among the cells there are primary gland cavities and the desmosome. The type C oncovirus particles are discovered among the carcinoma cells and in their cytoplasm, which are 100nm in diameter, in the shape of globe and with external envelopes endomembranes and central cores. Conclusion] This is the first time that rat spontaneous mammary adenocarcinomas have been established to generate type C oncovirus tumor strain. It makes up a type of rat mammary epithelial anaplastic adenocarcinomas. And its pathological morphology is of polymorphosm.