猿猴疱疹病毒B糖蛋白D的表达及抗原性的探讨

目的表达猴B病毒糖蛋白D(BVgD)并对其抗原性进行初步研究。方法应用体外原核表达BVgD,继而进行Ni离子亲和层析纯化。采用Western blotting对蛋白抗原性进行分析,评估其抗原潜力。结果完成了对BVgD的小量表达和纯化。Western blotting结果表明表达出的重组蛋白BVgD具有特异性结合猴B病毒抗体的能力。结论BVgD重组蛋白具有良好的抗原性,与HSV-1抗体无交叉反应。具有进一步开发的潜力,也为BVgD免疫原性等方面的进一步研究提供了条件。

Preliminary Study on the Expression of Monkey B Virus Glycoprotein D and Its Antigenicity

Objective To study the express of monkey B virus glycoprotein D in E-coli cells and detect its antigenicity.Methods BVgD was expressed in E-coli and than purified.The antigenic potential of BVgD was evaluated by Western blotting.Result The recombinant protein BVgD was successfully expressed and purified,and the result of Western blotting showed that the recombinant BVgD had good specificity and sensitivity to react with antibodies to monkey B virus.Conclusion The expressed recombinant protein has no cross reaction with HSV-1 positive serum.This protein is worthy of further development and provides valuable information for research of immunogenicity of BVgD.