紫外分光光度法测定转移因子透析液中多肽含量

 以凯氏定氮法测定结果为基础,采用紫外分光光度法测定转移因子透析液中的多肽含量?该法的回收率及相对标准偏差分别为99.56%?1.48%(n=6);经与Folin-酚法相比较,确定两种方法测得含量的比值为7.17,RSD为3.77%(n=20)?相关系数为0.93;并推算出当吸收值为15.10时,多肽含量为1.0mg/ml?从而为转移因子透析液中多肽的定量提供了一个快速?简便?准确的方法?

The determination of concentrations of polypeptide in transfer factorextractive by using ultraviolet spectrophotometry

Ultraviolet spectrophotometry(UVS)for the determination of polypeptide in thetransfer factor(TF)extractive was established. The concentration of polypeptide in TF extractivewas first detected with kjeldahl-gunning method for nitrogen determination.Basedon the resultsablained,the standard cuvre of UVS was laid down.The recovery of UVS was 99.56%±1.48%(n=6). The results of UVS were well correlated with those of Folin-phenol method(r=0. 93,n=20). The ratio of the concentrations determined by the two methods,CUV/CF was 7. 17±3. 77%.Itwas showed that the concentration of polypeptide is 1.0mg/ml when the UV absorbance of TFextractive is 15. 10. This study provides a convenient ,rapid and quantitative method for the deter-mination of TF extractive.