软骨肉瘤细胞PTHrP和Sox9及Bcl-2基因表达及其相互作用的研究 |
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引用本文: | 秦宏敏,;韩会峰,;沙广钊,;彭易根,;任天成.软骨肉瘤细胞PTHrP和Sox9及Bcl-2基因表达及其相互作用的研究[J].齐鲁肿瘤杂志,2009(4):276-279. |
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作者姓名: | 秦宏敏 ;韩会峰 ;沙广钊 ;彭易根 ;任天成 |
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作者单位: | [1]徐州医学院附属医院骨科,江苏徐州221002; [2]邳州第一人民医院骨科,江苏邳州221300 |
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基金项目: | 江苏省自然科学基金(05KJB320135);江苏省高校自然科学研究基金(BK2005015) |
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摘 要: | 目的:研究PTHrP、Sox9和Bcl-2基因在人体软骨肉瘤细胞HTB-94中的相互作用机制。方法:小干扰siRNA阻断PTHrP后,分别检测肿瘤细胞中Sox9、Bcl-2和Col2α1的mRNA及蛋白表达。结果:PTHrP基因被干扰后,PTHrP基因的mRNA及蛋白表达降低,Sox9、Bcl-2和Col2α1的mRNA及蛋白表达都受到抑制;而PTHrP基因的干扰作用去除,Sox9、Bcl-2和Col2α1的mRNA及蛋白表达抑制作用也消失。结论:PTHrP基因在肿瘤细胞的抑制引起Sox9、Bcl-2和Col2α1肿瘤相关基因的抑制,提示PTHrP基因是Sox9、Bcl-2和Col2α1肿瘤相关基因的上游基因。
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关 键 词: | 软骨肉瘤 RNA干扰 基因表达 印迹法 蛋白质 |
Experimental research on relationship among PTHrp,Sox9 and Bcl-2 genes in chondrosarcoma cell line |
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Institution: | QIN Hong-min , HAN Hui- feng , SHA Guang-zhao , PENG Yi-gen , REN Tian-cheng( 1. Department of Orthopedic, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, P. R. China 2. Department of Orthopedic, First People's Hospital of Pizhou, Pizhou 221300, P. R. China) |
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Abstract: | OBJECTIVE: To study the functional mechanism among PTHrp, Sox9 and Bcl-2 genes in chondrosarcoma cell line. METHODS: The Sox9, Bcl-2 and Col2α1 mRNA and protein expressions were tested after siRNA interfering the gene of PTHrp. RESULTS: After the interference of PTHrP, the expressions of mRNA and proteins from PTHrP, Sox9, Bcl-2 and Col2α1 were all reduced,but without the interference of PTHrp, Sox9, Bcl-2 and Col2α1 were all in the normal behavior. CONCLUSIONS: The inhibition of PTHrp could inhibit the Sox9, Bcl-2 and Col2α1 genes. PTHrp might be the upper stream gene in the rest of Sox9, Bcl-2 and Co12α1 genes. |
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Keywords: | chondrosarcoma RNA interference gene expression blotting western |
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