脊髓损伤后骨质疏松小鼠的骨折愈合

目的 通过脊髓损伤小鼠和对照组小鼠骨折愈合的对比研究来了解神经因素在骨折愈合中的作用.方法 将120只脊髓损伤小鼠(SCI)和对照组小鼠(CON)做成单侧股骨骨折模型.在术后2、4和8w,每个时间点,20只(SCI:10只,CON:10只)通过硅酮橡胶(microfil)灌注后micro-CT检查骨痂微血管,血清检测VEGF了解骨愈合过程中的血管形成的情况,检测骨钙素、碱性磷酸酶了解骨折愈合情况,同时另外20只进行骨密度,骨痂微结构分析,生物力学检测.结果 脊髓损伤小鼠骨折愈合中中血管形成情况,骨密度,骨痂微结构和生物力学都明显弱于对照组,血清VEGF、骨钙素、碱性磷酸酶也明显弱于对照组.结论 脊髓损伤明显抑制了骨折愈合,减少骨折处局部骨痂量,神经因素在骨折愈合过程中扮演着重要角色.

Fracture healing of osteoporotic mice after spinal cord injury

Objective To explore the role of neural factors on the fracture healing through the comparative research between spinal cord injury (SCI) mice and control (CON) mice. Methods One hundred and twenty mice were made of unilateral femur fracture model, including 60 SCI mice and 60 CON mice. On 2, 4 and 8 weeks after the fracture, neovascularization was observed by vascular perfusion with Microfil in micro-CT, and serum VEGF was measured for learning vascular formation during fracture healing, and osteocalcin and alkaline phosphatase were measured for learning fracture healing, in 20 mice (10 of SCI, 10 of CON). The other 20 mice were under bone mineral density, micro-CT callus, and biomechanical property inspection. Results The angiogenesis, BMD, callus microarchitecture, and biomechanical properties of SCI mice were significantly weaker than those in control group, as well as serum VEGF, osteocalcin, and alkaline phosphatase. Conclusion SCI significantly inhibits fracture healing, reduces the amount of callus. Neural factor plays an important role in the fracture healing.