RP-HPLC法测定银天颗粒中主要药效成分的含量

目的:建立测定银天颗粒中绿原酸和栀子苷含量的方法。方法:采用反相高效液相色谱法。绿原酸检测色谱条件:色谱柱为Platisil-ODS(250mm×4.6mm,5μm),流动相为乙腈-0.2%磷酸(20:80),检测波长为326nm,流速为1.0mL·min-1,柱温为25℃。栀子苷检测色谱条件:色谱柱为AgilentZorbaxSB-C18(250mm×4.6mm,5μm),流动相为乙腈-水(10:90),检测波长为238nm,流速为1.0mL·min-1,柱温为25℃。结果:绿原酸、栀子苷的检测浓度分别在5.4～108.0、11～110μg·mL-1范围内与各自峰面积积分值呈良好线性关系(r均为0.9999);平均回收率分别为99.04%(RSD=2.66%,n=6)和97.69%(RSD=2.07%,n=6)。结论:本方法专属性强、重复性好、结果准确,可用于银天颗粒的质量控制。

Content Determination of Main Active Components in Yintian Granules by RP-HPLC

OBJECTIVE:To establish methods for the content determination of chlorogenic acid and gasminoidin in Yintian granules.METHODS:RP-HPLC method was adopted.The chromatographic conditions of chlorogenic acid were as follows:Platisil-ODS(250 mm×4.6 mm,5 μm)column was used with mobile phase consisted of acetonitrile-0.2% phosphoric acid(20:80)at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 326 nm.The column temperature was 25 ℃.The chromatographic conditions of gasminoidin were as follows:Agilent Zorbax SB-C18(250 mm×4.6 mm,5 μm)column was used with mobile phase consisted of acetonitrile-water solution(10:90)at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 238 nm and column temperature was 25 ℃.RESULTS:Chlorogenic acid showed a good linear relationship at range of 5.4～108.0 μg·mL-1(r=0.999 9)with an average recovery of 99.04%(RSD=2.66%,n=6).Gasminoidin showed a good linear relationship at range of 11～110 μg·mL-1(r=0.999 9)with an average recovery of 97.69%(RSD=2.07%,n=6).CONCLUSION:The methods are specific,reproducible and accurate,and can be used for the quality control of Yintian granules.