UPLC-MS/MS测定降压类中成药及保健品中30种化学药

 目的 建立一种测定降压类中成药及保健品中添加30种化学药的超高效液相-串联四级杆质谱方法。方法 多反应监测模式测定，以Waters ACQUITY UPLC BEH C₁₈柱进行分离，甲醇-0.1%甲酸溶液梯度洗脱，流速：0.2 mL·min^-1。离子化模式：ESI⁺。结果 30种化学药测定的线性范围分别在10～1 000.0， 0～400.0， 2.0～400.0 μg·L^-1，r分别在0.990 3～0.999 8之间，检出浓度分别在0.01~0.83 μg·L^-1之间， 3个水平的回收率分别在72.2%～123.4%之间。结论 该方法快速、准确、灵敏，可用于降压类中成药及保健品中添加30种化学药的测定。

Determination of Thirty Antihypertensive Chemical Medicines Adulterateds in Traditional Chinese Patent Medicines and Health Foods by UPLC-MS/MS

OBJECTIVE To establish a method for determination of 30 antihypertensive medicines adulterated in traditional Chinese patent medicines and health foods by UPLC triple-quadrupole tandem mass spectrometer.METHODS Multi-reactions monitoring(MRM) technology was used.The separation was performed on a Waters ACQUITY UPLCTM BEH C18 column with a gradient mobile phase of methanol-0.1% formic acid at flow rate of 0.2 mL·min-1.The ionization mode was ESI+.RESULTS The standard curves of the 30 chemical medicines showed good linearity over the concentration ranges of 10-1 000.0,0-400.0 and 2.0-400.0 μg·L-1(r=0.990 3-0.999 8).The detection limits of the 30 chemical medicines were between 0.01-0.83 μg·L-1 and the average recoveries were 72.2%-123.4% at 3 levels.CONCLUSION The method is rapid and sensitive,and suitable for the determination of 30 chemical medicines in traditional Chinese patent medicines and health foods.