血管紧张素Ⅱ1型受体基因多态性与高龄原发性高血压的相关性

目的研究血管紧张素Ⅱ1型受体(AT1R)基因A1166C多态性与汉族高龄原发性高血压的相关关系。方法采用PCR-RFLP方法,对山东省章丘地区高龄原发性高血压患者104例(高龄高血压组)和体检正常老年人98例(正常对照组)的外周血白细胞DNA,进行AT1R基因A1166C多态性检测,观察AA、AC和CC不同基因型以及该点A、C不同等位基因频率在两组中的分布。结果AA、AC和CC基因型在高龄高血压组的分布为83(79.81%)、20(19.23%)和1(0.96%),正常对照组为82(83.67%)、16(16.33%)和0,两组比较差异无显著性意义(χ2=0.50,P>0.05);A1166与C1166等位基因频率在高龄高血压组中分别为186(89.42%)和22(10.58%),正常对照组为180(91.84%)和16(8.16%),两组比较差异无显著性意义(χ2=1.38,P>0.05)。结论AT1R基因A1166C分子变异与山东省章丘地区汉族高龄原发性高血压无相关性。

Relationship between polymorphism of AT1 receptor gene and the essential hypertension in very old patients

Objective To investigate the relationship between the A1166C allele polymorphism of angiotensinⅡtype 1 receptor(AT1R)gene and the essential hypertension in very old patients.Methods Polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP) methods were used to detect the polymorphism of AT1R gene in 104 very old patients with hypertension and 98 normotensive controls.Results The genotype frequencies of A1166C were AA83(79.81%),AC20(19.23%) and CC1(0.96%) in very old patients group and the corresponding frequencies were 82(83.67%),16(16.33%) and 0 in the control group respectively.The A1166 and C1166 allele frequencies were 186(89.42%) and 22(10.58%) respectively in very old patients group and 180(91.84%) and 16(8.16%) in normotensive group.The A1166C genotype distribution and the allele frequencies of A1166 and C1166 had no statistically significant difference in very old patients group compared with normotensive group(P>0.05).Conclusion There was no significant association between the A1166C polymorphism of AT1R gene and the essential hypertension in very old patients in Zhangqiu of Shandong province.