约氏疟原虫感染大鼠和小鼠的红外期研究

约氏疟原虫约氏亚种的子抱子经尾静脉接种大鼠和三株小鼠(ICR/JCL,C57BL,KM株)后42h,在大鼠和小鼠的肝连续切片中均查见红外期裂殖体(EE裂殖体),但在KM鼠株的切片中,正常的EE裂殖体甚少。不论宿主同否,EE裂殖体均大小不等,呈圆形、椭圆形或其他形状,分化程度不同,发育不完全同步。大鼠肝中的EE裂殖体可看到明显的外膜,周围并出现细胞反应。ICR/JCL、C57BL鼠株和大鼠肝中的EE裂殖体均较KM鼠株发育良好,数量较多,但大鼠中的红内期迅速消失。结果表明;ICR/JCL和C57BL鼠株用作约氏疟原虫—斯氏按蚊系统鼠疟模型的宿主较合适。

STUDY ON THE EXOERYTHROCYTIC FORMS OF PLASMODIUM YOELII YOELII IN RATS AND MICE

SD rats and three strains of mice were infected with Plasmodium yoelii yoelii By265 strain by intravenous inoculation of sporozoites via tail vein. Liver tissues were taken 42 hours after infection and serial sections were made and stained by Colophonium-Giemsa method for microscopic examination. The ratio of the average value of major diameter/minor diameter of exoerythrocytic(EE)schizonts of Plasmodium yoelii yoelii in rats and mice of ICR/JCL, C57BL, KM strains was 35.81 +/- 4.56 microns/29.72 +/- 4.08 microns, 28.08 +/- 4.66 microns/23.66 +/- 4.44 microns, 28.14 +/- 4.16 microns/23.63 +/- 3.77 microns, 23.80 +/- 2.42 microns/21 +/- 0 microns, respectively. The results showed that the development of EE schizonts of Plasmodium yoelii yoelii was not synchronous. The EE schizonts in the rat liver were surrounded by Kupffer cells, monocytes and monocyte-derived macrophages. Since the parasitemia disappeared rapidly in rats, and EE schizonts were not well developed in KM strain, it may be considered that ICR/JCL and C57BL strains are more suitable as vertebrate host in Plasmodium yoelii yoelii-Anopheles stephensi system model (Figs. 1-9).