应激对豚鼠牙周炎模型的影响

目的探讨应激对豚鼠牙周炎模型的影响。方法龈沟接种伴放线放线杆菌和牙龈卟啉单胞菌,制备豚鼠牙周炎模型24只,随机分为应激组12只(冷刺激和惊吓刺激)和对照组12只(无刺激),于1、2、4、6周后分批处死,进行临床参数、病理切片、破骨细胞和成骨细胞计数以及血清皮质醇浓度检测。结果应激组第1、2、4周皮质醇浓度升高,第2、4周牙周袋深度与对照组比较有明显差异(P<0.05或P <0.01)。病理切片显示:与对照组相比,应激组牙周破坏更明显,骨修复不活跃,应激组第1、4、6周破骨细胞计数明显增高(P<0.05)。结论应激加重致病菌感染后牙周组织的破坏,延缓组织修复,是牙周炎的重要危险因子。

The Effect of Stress on Periodontitis Model of Guinea Pigs

Objective To investigate the effect of stress on Guinea Pigs'periodontitis model.Methods 24periodontitis models of guinea pigs were provided by inoculating Actinobacillus actinomycetemcomitans(Aa)and Porphyromonas gingivalis(Pg)in the gingival sulcus.The guinea pigs were divided into two groups:stress group(stimulated with cold bath and fright)and control group(without stimulations).Three animals of each group were sacrificed successively at1,2,4,6week after inoculation.Clinical and histological evaluation,cortisol concentration examinations,osteoclast and osteoblast numbers account were applied.Results The cortisol concentration of stress group was significantly higher than that of control group at1,2,4week after stressed(P<0.05or P<0.01).Pocket depth in stress group was significantly deeper than that in control group at2,4week(P<0.05),Compared with control group,the stress group demonstrated more active tissue damage and alveolar bone resorption.The bone repair in stress group was not as active as in control group.The osteoclast numbers in stress group was significantiy higher than that in control group at1,4,6week(P<0.05).Conclusions:Stress could enhance the destruction of periodontal tissues infected by pathogenic bacteria and increase disease susceptibility,It was concluded that stress was a significant risk indicator for periodontitis.